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master's thesis
Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:063064
Buovac, Marko
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Buovac, M. (2024). Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:063064

Buovac, Marko. "Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:063064

Buovac, Marko. "Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:063064

Buovac, M. (2024). 'Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:063064

Buovac M. Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2025 January 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:063064

M. Buovac, "Utjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:063064

Metadata
TitleUtjecaj izvora i koncentracije dušika na rast i proizvode metabolizma kvasca Kluyveromyces marxianus NBRC 1777
Title (english)Influence of nitrogen source and concentration on growth and products of metabolism of yeast Kluyveromyces marxianus NBRC 1777
AuthorMarko Buovac
MentorVlatka Petravić Tominac (mentor)
MentorNenad Marđetko (pomoć pri izradi, neposredni voditelj)
Committee memberMario Novak (predsjednik povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
Committee memberJasna Mrvčić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2024-03-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Istražen je utjecaj izvora i koncentracije dušika na kvasac K. marxianus NBRC 1777. Uzgojem na triptonu kao jedinom izvoru dušika, najveći prinos biomase (1,99 g L⁻¹) i koeficijent konverzije supstrata (glukoze) u biomasu (0,20 g g⁻¹) postignuti su uzgojem na podlozi sastava 22,73 g L⁻¹ glukoze i 7,5 g L⁻¹ triptona, dok je najveći prinos etanola (2,36 g L⁻¹) ostvaren uzgojem na podlozi s 22,73 g L⁻¹ glukoze i 6 g L⁻¹ triptona. Zamjenom dijela triptona amonijevim solima uglavnom su se smanjili prinos biomase i proizvoda metabolizma. Međutim, uzgojem na podlozi s 45,46 g L⁻¹ glukoze, 5 g L⁻¹ triptona i 2,5 g L⁻¹ amonijevog hidrogenfosfata i amonijevog sulfata ostvaren je prinos biomase 2,59 g L⁻¹ . Kukuruzna močevina pokazala se kao najbolji izvor dušika za korišteni mikroorganizam pri istraženim uvjetima, jer je jedino njezinom primjenom radni mikroorganizam skoro potpuno potrošio glukozu, a postignuti su i najveći prinosi etanola. Najviše etanola (17,45 g L⁻¹) proizvedeno je u podlozi s 45,46 g L⁻¹ glukoze i 20 g L⁻¹ kukuruzne močevine.
Abstract (english)
The influence of the source and concentration of nitrogen on the yeast K. marxianus NBRC 1777 was investigated. With tryptone as a sole nitrogen source, the highest biomass yield (1.99 g L⁻¹) and the highest conversion coefficient of substrate (glucose) to biomass (0.20 g g⁻¹) were achieved by growing on a substrate containing 22.73 g L⁻¹ of glucose and 7.5 g L⁻¹ of tryptone, while the highest ethanol yield (2.36 g L-1) was achieved by cultivation on medium containing 22.73 g L⁻¹ of glucose and 6 g L⁻¹ of tryptone. By replacing part of tryptone with ammonium salts, the yields of biomass and metabolic products mainly decreased. However, growth in medium containing 45.46 g L⁻¹ glucose, 5 g L⁻¹ tryptone and 2.5 g L⁻¹ ammonium hydrogen phosphate and ammonium sulphate resulted in a biomass yield of 2.59 g L-1. Corn steep liquor (CSL) proved to be the best nitrogen source for the tested strain under the investigated conditions, since the working microorganism consumed glucose almost completely and the highest ethanol yields were achieved with CSL as the only nitrogen source. The highest ethanol concentration (17.45 g L⁻¹) was produced in medium containing 45.46 g L⁻¹ of glucose and 20 g L⁻¹ of CSL.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:063064
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-07-25 11:40:20