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master's thesis
Alkalna predobrada i enzimska hidroliza trstike
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:607538
Češković, Dorotea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Češković, D. (2024). Alkalna predobrada i enzimska hidroliza trstike (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:607538

Češković, Dorotea. "Alkalna predobrada i enzimska hidroliza trstike." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:607538

Češković, Dorotea. "Alkalna predobrada i enzimska hidroliza trstike." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:607538

Češković, D. (2024). 'Alkalna predobrada i enzimska hidroliza trstike', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:607538

Češković D. Alkalna predobrada i enzimska hidroliza trstike [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:607538

D. Češković, "Alkalna predobrada i enzimska hidroliza trstike", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:607538

Metadata
TitleAlkalna predobrada i enzimska hidroliza trstike
Title (english)Alkaline pretreatment and enzyme hydrolysis of reed
AuthorDorotea Češković
MentorAntonija Trontel (mentor)
Committee memberMario Novak (predsjednik povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
Committee memberBlaženka Kos (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2024-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu provedena je alkalna predobrada trstike (γ0 = 40 g L⁻¹; wNaOH = 1 – 6 %) pri relativno niskoj temperaturi od 60 °C tijekom 3 h, kao i alkalna predobrada trstike (wNaOH = 1 %) u visokotlačnom reaktoru pri temperaturi od 180 °C tijekom 10 minuta. Za hidrolizu predobrađene trstike korišteni su komercijalni enzimi u liofiliziranom obliku (Cellulase from Aspergillus niger, Hemicellulase from Aspergillus niger) i u tekući enzimi (Cellulase, enzyme blend, Viscozyme L). Ispitivanje učinkovitosti enzimske hidrolize provedeno je pri temperaturama od 35, 40 i 45 °C pri kojima može rasti veliki broj mikroorganizama producenata različitih biokemikalija i biogoriva. Odabir ovih temperatura za provođenje procesa hidrolize omogućuje jednostavnije uspostavljanje procesa simultane saharifikacije i fermentacije. Učinkovitost hidrolize glukana i ksiliana dostupnih u predobrađenoj trstici bila je u rasponu od 60 do 100 %, a hidroliza glukana i ksilana od 100 % postignuta je kod hidrolize s 1 mL Cellulase, enzyme blend i 1,5 mL Viscozyme L.
Abstract (english)
In this work, alkaline pretreatment of reed (γ0 = 40 g L⁻¹; wNaOH = 1 – 6 %) was carried out at a relatively low temperature of 60 °C for 3 hours, as well as alkaline pre-treatment of reed (wNaOH = 1%) in a high pressure reactor at a temperature of 180 °C for 10 minutes. Commercial enzymes in lyophilized form (Cellulase from Aspergillus niger, Hemicellulase from Aspergillus niger) and liquid enzymes (Cellulase, enzyme blend, Viscozyme L) were used for the hydrolysis of pre-treated reed. The effectiveness of enzymatic hydrolysis was tested at temperatures of 35, 40, and 45 °C, at which a large number of microorganisms producing various biochemicals and biofuels can grow. The selection of these temperatures for carrying out the hydrolysis process facilitates the implementation of simultaneous saccharification and fermentation processes. The efficiency of hydrolysis of glucans and xylans available in the pre-treated reed was from 60 to 100 %, with 100 % hydrolysis of glucans and xylans achieved with 1 mL of Cellulase, enzyme blend, and 1.5 mL of Viscozyme L.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:607538
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-10-16 10:23:21