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master's thesis
Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:096138
Miškulin, Ena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Miškulin, E. (2014). Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:096138

Miškulin, Ena. "Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:096138

Miškulin, Ena. "Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:096138

Miškulin, E. (2014). 'Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:096138

Miškulin E. Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:096138

E. Miškulin, "Utjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:096138

Metadata
TitleUtjecaj omjera izvora ugljika i dušika u podlozi na proizvodnju lipida u kvascu Trichosporon oleaginosus
Title (english)Influence of the carbon to nitrogen source ratio in the medium on lipid production with yeast Trichosporon oleaginosus
AuthorEna Miškulin
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2014-07-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Proveden je kontinuirani uzgoj kvasca Trichosporon oleaginosus s podlogom za prihranu koja je sadržavala glukozu kao izvor ugljika i amonijev klorid kao izvor dušika. Proučavan je utjecaj omjera ugljika i dušika (C/N, g g-1) u podlozi za prihranu na koncentraciju biomase i udjele proteina i lipida u stanici pri konstantnoj brzini rasta (D = 0,1 h-1). Na osnovu potrošnje supstrata i sastava stanice uočena su tri karakteristična tipa rasta. Pri malim omjerima C/N (≤ 15 g g-1) rast stanice limitiran je izvorom ugljika, dok je izvor dušika u suvišku (visoki udjel proteina i mali udjel lipida u stanici). Pri uzgoju s podlogom za prihranu sastava 15 g g-1 < C/N ≤ 46 g g-1, izvor dušika i ugljika bio je potpuno utrošen. Tijekom limitacije rasta s dva supstrata udjel proteina u stanici se smanjio, dok se udjel lipida povećao. Daljnjim povećanjem omjera C/N iznad 46 g g-1 dolazi do limitacije rasta stanica izvorom dušika, dok koncentracija izvora ugljika raste iznad 1 g L-1 (mali udjel proteina i veliki udjel lipida). Tijekom limitacije rasta izvorom dušika uz omjer C/N 153 g g-1 postignut je najveći prinos lipida od 0,27 g g-1 i najveća specifična brzina sinteze lipida od 0,0998 g g-1 h-1.
Abstract (english)
The oleaginous yeast Trichosporon oleaginosus was grown in continuous culture with feed medium containing glucose as carbon source and ammonium chloride as nitrogen source. The influence of the carbon : nitrogen ratio (C/N, g g-1) of feed medium on biomass concentration and protein and lipid content in the cell was studied at a constant growth rate (D = 0,1 h-1). On the basis of substrates utilisation and cell composition three characteristic growth paths were observed. At low C/N ratio (≤15 g g-1) cell growth was carbon-limited and nitrogen source was present in excess (high protein and low lipid content in cell). Growth with a medium of 15 g g-1 < C/N ≤ 46 g g-1resulted in complete utilisation of nitrogen and carbon source in the medium. During double-substrate limited growth, protein content was reduced while lipid content was increased. Further increase of C/N ratio resulted in nitrogen-limited growth, while carbon source concentration increased above 1 g L-1 (low protein and high lipid content). Under nitrogen limitation at the C/N ratio of 153 g g-1, the highest lipid yield of 0,27 g g-1 and the highest specific lipid formation rate of 0,0998 g g-1 h-1was observed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:096138
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-04-28 11:55:11