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master's thesis
In silico karakterizacija metagenomskih alkan 1-monooksigenaza
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:068577
Oršolić, Davor
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Oršolić, D. (2015). In silico karakterizacija metagenomskih alkan 1-monooksigenaza (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:068577

Oršolić, Davor. "In silico karakterizacija metagenomskih alkan 1-monooksigenaza." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:068577

Oršolić, Davor. "In silico karakterizacija metagenomskih alkan 1-monooksigenaza." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:068577

Oršolić, D. (2015). 'In silico karakterizacija metagenomskih alkan 1-monooksigenaza', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:068577

Oršolić D. In silico karakterizacija metagenomskih alkan 1-monooksigenaza [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:068577

D. Oršolić, "In silico karakterizacija metagenomskih alkan 1-monooksigenaza", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:068577

Metadata
TitleIn silico karakterizacija metagenomskih alkan 1-monooksigenaza
Title (english)In silico characterisation of metagenomic alkane 1-monooxygenases
AuthorDavor Oršolić
MentorJurica Žučko (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2015-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj istraživanja bio je provesti filogenetsku analizu, funkcionalnu karakterizaciju i trodimenzionalno strukturno modeliranje hipotetskih alkan monooksigenaza iz metagenomske knjižnice sastavljene iz umjereno onečišćenog uzorka sakupljenog iz sedimenta lučnog sidrišta u Puli. Filogenetska analiza provedena je s pomoću MEGA programa, te je dala hipotetski prikaz evolucijske povezanosti između proteina. Funkcionalnom karakterizacijom putem UniProt, InterPro i CD baze podataka potvrđena je pripadnost ispitivanih proteina alkan 1-monooksigenazama, dok strukturno modeliranje s pomoću SWISS-MODEL i I-TASSER web servisa nisu dali značajne rezultate niti za jedan od proteina. Neuspjelost strukturnog modeliranja pripisana je nedostatku homolognih proteina s eksperimentalno određenom trodimenzionalnom strukturom.
Abstract (english)
Phylogenetic analysis, functional characterization and structural modeling of putative alkan monooxygenases from metagenomic library constructed from a moderately polluted sample collected from a tanker berth station in Pula was performed. Phylogenetic analysis conducted via MEGA software resulted in phylogenetic tree representing hypothesis of evolutionary relationships between proteins. Functional characterization using UniProt, InterPro and CDD search engines confirmed similarities between putative proteins and alkan 1-monooxygenases, while structural modeling via SWISS-MODEL and I-TASSER server didn't give significant results. Inefficiency of structural modeling was attributed to absence of homologous proteins with experimentally determined three-dimensional structure.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:068577
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 12:40:09