prikaz prve stranice dokumenta Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A
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master's thesis
Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:540934
Pugelnik, Jelena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for General Microbiology and Food Microbiology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Pugelnik, J. (2014). Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:540934

Pugelnik, Jelena. "Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:540934

Pugelnik, Jelena. "Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:540934

Pugelnik, J. (2014). 'Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:540934

Pugelnik J. Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:540934

J. Pugelnik, "Plemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:540934

Metadata
TitlePlemenita plijesan Penicillium nalgiovense kao mogući producent aflatoksina B1 i okratoksina A
Title (english)Noble mould Penicillium nalgiovense as possible producent of aflatoxin B1 and ochratoxin A
AuthorJelena Pugelnik
MentorKsenija Markov (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for General Microbiology and Food Microbiology)
Zagreb
Defense date and country2014-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Plemenita plijesan Penicillium nalgiovense ima biotehnološku važnost u prehrambenoj industriji (suhomesnati proizvodi) zbog svoje široke upotrebe kao starter kultura. Obzirom da plijesni sintetiziraju sekundarne toksične metabolite mikotoksine, suhomesnati proizvodi predstavljaju potencijalnu opasnost po zdravlje potrošača. Svrha ovog rada bio je istražiti može li liofilizirana kultura plemenite plijesni Penicillium nalgiovense i soj plijesni Penicillium sp. 622 koje se koriste u industrijskoj proizvodnji zimske salame, pri odabranim uvjetima uzgoja, sintetizirati aflatoksin B1 (AFB1) i okratoksin A (OTA). Za istraživanje prisutnosti mikotoksina u uzorcima upotrijebljena je tankoslojna kromatografija, a koncentracija AFB1 i OTA je određena ELISA (Enzyme Linked Immuno Sorbent Assay) metodom. Rezultati istraživanja su pokazali da je AFB1 dokazan u 72,7 %, a OTA u 100% istraživanih uzoraka. Koncentracije AFB1 bile su u rasponu od nd (nije dokazano) do 0,125 pg×mL-1, dok je najveća količina OTA od 4,0 pg×mL-1 dokazana u liofilizatu komercijalne starter kulture P. nalgiovense.
Abstract (english)
The noble mold Penicillium nalgiovense has a biotechnological importance in the food industry ( meat products ), because of its wide use as starter culture. Since mold synthesizes toxic secondary metabolites, mycotoxins, meat products poses a potential threat to abusers health. The purpose of this paper was to investigate whether the lyophilized cultures of noble Penicillium nalgiovense and strain of Penicillium sp. 622, which are used in the industrial production of winter salami, in chosen growing conditions, synthesizes aflatoxin B1 (AFB1) and ochratoxin A (OTA). The presence of mycotoxins in the samples was determined by thin layer chromatography, and the concentration of AFB1 and OTA was determined by qualitative ELISA ( Enzyme Linked Immuno Sorbent Assay ) method. The study results showed that AFB1 was detected in 72.7% and OTA in 100% of the samples. AFB1 concentrations ranged from ND (not detected ) to 0,125 pg × ml-1, while the maximum amount of OTA (4.0 pg × ml-1) was detected in the lyophilized commercial starter culture of P. nalgiovense.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:540934
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 12:54:15