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master's thesis
Određivanje ostataka flumekina u mlijeku ELISA metodom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:876252
Vrebac, Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vrebac, M. (2014). Određivanje ostataka flumekina u mlijeku ELISA metodom (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:876252

Vrebac, Marija. "Određivanje ostataka flumekina u mlijeku ELISA metodom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:876252

Vrebac, Marija. "Određivanje ostataka flumekina u mlijeku ELISA metodom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:876252

Vrebac, M. (2014). 'Određivanje ostataka flumekina u mlijeku ELISA metodom', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 October 2024, https://urn.nsk.hr/urn:nbn:hr:159:876252

Vrebac M. Određivanje ostataka flumekina u mlijeku ELISA metodom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 October 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:876252

M. Vrebac, "Određivanje ostataka flumekina u mlijeku ELISA metodom", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:876252

Metadata
TitleOdređivanje ostataka flumekina u mlijeku ELISA metodom
Title (english)Determionation of flumenquine residues in milk by ELISA method
AuthorMarija Vrebac
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2014-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Zbog raširene primjene antibiotika u veterinarskoj medicini određivanje rezidua antibiotika u uzorcima životinjskog podrijetla, postalo je nužnim u svrhu zaštite krajnjih potrošača i sprječavanja širenja antibiotičke rezistencije. Kontrola ostataka antibiotika i najveće dopuštene količine (NDK) antibiotika u mlijeku regulirane su na razini Europske unije. U državama Europske unije u veterinarske svrhe potroši se 3494 tone antimikrobnih lijekova, od čega su 43 tone fluorokinoloni. Cilj ovog rada bio je odrediti koncentracije ostataka (rezidua) flumekina u različitim uzorcima mlijeka. Koncentracija flumekina u uzorcima je određivana ELISA metodom. Prema dobivenim rezultatima, niti u jednom ispitanom uzorku određena koncentracija flumekina nije prelazila vrijednost najveće dopuštene količine (NDK) za flumekin propisane Uredbom Europske komisije 37/2010 o najvećoj dopuštenoj količini (NDK) ostataka antibiotika u hrani životinjskog podrijetla. Također je pokazano da je ELISA pouzdana metoda za određivanje rezidua flumekina u uzorcima hrane životinjskog podrijetla. Da su uzorci bili pozitivni na flumekin, u tom bi slučaju bilo potrebno primjeniti jednu od metoda za potvrdu kao što su LC–MS/MS i GC–MS/MS.
Abstract (english)
Due to the widespread usage of antibiotics in veterinary medicine, determination of antibiotics residues in samples of animal origin has become necessary in order to protect the consumers and prevent the spread of antibiotic resistance. The control of antibiotic residues and maximum residue limit (MRL) of antibiotics in milk are regulated at EU level. 3,494 tons of antimicrobial drugs, out of which 43 tons fluoroquinolones are consumed in European Union countries for veterinary purposes. The aim of this study was to determine the concentrations of flumekin remains (residues) in different milk samples. The concentration of flumekin in the samples was determined by ELISA method. According to the results, in none of the samples examined did the concentration of flumekin exceed the value of the maximum residue limit (MRL) for flumekin prescribed by the Commission Regulation 37/2010 on the maximum residue limit (MRL) of antibiotic residues in food of animal origin. It is also shown that the ELISA is a reliable method for determination of flumekin residues in food samples of animal origin. If the samples were positive for flumekin, it would be necessary to apply one of the methods for verification such as LC-MS / MS and GC-MS / MS
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:876252
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 07:55:14