prikaz prve stranice dokumenta Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles
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master's thesis
Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:152048
Mesihović, Anida
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Mesihović, A. (2013). Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović, Anida. "Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović, Anida. "Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović, A. (2013). 'Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović A. Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:152048

A. Mesihović, "Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:152048

Metadata
TitleUltrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles
AuthorAnida Mesihović
MentorVladimir Mrša (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2013-07-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ultrasound and microbubbles-mediated gene transfer is a controlled DNA delivery technique that is less invasive and more effective at targeted delivery than other currently available approaches. The main limitation of this technique is the low transfection efficiency of the commercially available microbubbles. Cationic microbubbles (MBs) were synthesized with the aim of improving their DNA binding capacity and their gene transfer. Two microbubble families were produced using two kinds of lipids available at the laboratory. The second family of microbubbles showed required properties: a proper stability, a good size distribution and acoustic activity. Zeta potential and DNA binding capacity were not as high as expected. Nevertheless, in vitro sonoporation of HeLa cells using the produced microbubbles resulted in almost 30% of transfection efficiency. We also performed in vivo gene transfer and demonstrated that a stable luciferase expression lasting for two weeks after sonoporation was achieved using our microbubbles.
Keywords
Languageenglish
URN:NBNurn:nbn:hr:159:152048
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-05 08:19:48