prikaz prve stranice dokumenta Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles
Pristup korisnicima matične ustanove
diplomski rad
Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles
Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2013. urn:nbn:hr:159:152048
Mesihović, Anida
Sveučilište u Zagrebu
Prehrambeno-biotehnološki fakultet
Zavod za kemiju i biokemiju
Laboratorij za biokemiju

Institucijski repozitorij: Repozitorij Prehrambeno-biotehnološkog fakulteta u Zagrebu

Citirajte ovaj rad

Mesihović, A. (2013). Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles (Diplomski rad). Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović, Anida. "Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles." Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2013. https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović, Anida. "Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles." Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2013. https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović, A. (2013). 'Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles', Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, citirano: 30.11.2024., https://urn.nsk.hr/urn:nbn:hr:159:152048

Mesihović A. Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles [Diplomski rad]. Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet; 2013 [pristupljeno 30.11.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:159:152048

A. Mesihović, "Ultrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles", Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, Zagreb, 2013. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:159:152048

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:159:152048
Podaci o radu
NaslovUltrasound and microbubbles-mediated gene transfer ; development of cationic microbubbles
AutorAnida Mesihović
MentorVladimir Mrša (mentor)
Ustanova koja je dodijelila
akademski / stručni stupanj		Sveučilište u Zagrebu
Prehrambeno-biotehnološki fakultet
(Zavod za kemiju i biokemiju)
(Laboratorij za biokemiju)
Zagreb
Datum i država obrane2013-07-01, Hrvatska
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Biotehnologija
Sažetak
Ultrasound and microbubbles-mediated gene transfer is a controlled DNA delivery technique that is less invasive and more effective at targeted delivery than other currently available approaches. The main limitation of this technique is the low transfection efficiency of the commercially available microbubbles. Cationic microbubbles (MBs) were synthesized with the aim of improving their DNA binding capacity and their gene transfer. Two microbubble families were produced using two kinds of lipids available at the laboratory. The second family of microbubbles showed required properties: a proper stability, a good size distribution and acoustic activity. Zeta potential and DNA binding capacity were not as high as expected. Nevertheless, in vitro sonoporation of HeLa cells using the produced microbubbles resulted in almost 30% of transfection efficiency. We also performed in vivo gene transfer and demonstrated that a stable luciferase expression lasting for two weeks after sonoporation was achieved using our microbubbles.
Ključne riječi
Jezikengleski
URN:NBNurn:nbn:hr:159:152048
Studijski programNaziv: Molekularna biotehnologija
Vrsta studija: sveučilišni
Stupanj studija: diplomski
Akademski / stručni naziv: magistar/magistra inženjer/inženjerka molekularne biotehnologije (mag. ing. biotechn.)
Vrsta resursaTekst
Način izrade datotekeIzvorno digitalna
Prava pristupaPristup korisnicima matične ustanove
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2017-05-05 08:19:48