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master's thesis
Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:084547
Zrnić, Klara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Zrnić, K. (2013). Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:084547

Zrnić, Klara. "Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:084547

Zrnić, Klara. "Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:084547

Zrnić, K. (2013). 'Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:084547

Zrnić K. Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:084547

K. Zrnić, "Usporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:084547

Metadata
TitleUsporedba ELISA testa i LC-MS/MS metode za određivanje aflatoksina M1 u mlijeku
Title (english)Comparation of ELISA test and LC-MS/MS method for determing of aflotoxin M1 in milk
AuthorKlara Zrnić
MentorIvana Radojčić Redovniković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2013-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
ELISA testom i metodom tekućinskom kromatografijom visoke djelotvornosti u kombinaciji sa spektrometrijom masa (LC-MS/MS) analiziran je uzorak mlijeka obogaćen aflatoksinom M1 i na temelju rezultata uspoređene te dvije analitičke metode. Određen je parametar iskorištenja, koeficijent varijacije, linearnost metode i matriksa te limit detekcije određivanja aflatoksina M1. Rezultati su pokazali veću točnost analize LC-MS/MS metodom. Zbog toga se ELISA test i koristi kao orijentacijska metoda, dok LC-MS/MS služi kao potvrdna metoda u slučaju pozitivnog testa uzorka.
Abstract (english)
The sample of milk enriched with aflatoksin M1 has been analyzed by ELISA test and high performance liquid chromatography with tandem mass spectrometry detection (LC-MS/MS) and the comparation of these two analytical methods has been carried out based on the results. Recovery, coefficient of variation, linearity of method and matrix and detection limit of aflatoksin M1 detection have been determined. The results have shown higher measurement accuracy by LC-MS/MS analysis. Thus, ELISA test is used as a comparation method and LC-MS/MS as an affirmative method when the result shows to be positive.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:084547
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-05 09:19:20