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master's thesis
Biotransformacija katehola u mikroreaktoru katalizirana lakazom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:131538
Đukić, Irena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Process Engineering
Laboratory for Measurement, Regulation and Automatisation

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Đukić, I. (2012). Biotransformacija katehola u mikroreaktoru katalizirana lakazom (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:131538

Đukić, Irena. "Biotransformacija katehola u mikroreaktoru katalizirana lakazom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:131538

Đukić, Irena. "Biotransformacija katehola u mikroreaktoru katalizirana lakazom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:131538

Đukić, I. (2012). 'Biotransformacija katehola u mikroreaktoru katalizirana lakazom', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:131538

Đukić I. Biotransformacija katehola u mikroreaktoru katalizirana lakazom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:131538

I. Đukić, "Biotransformacija katehola u mikroreaktoru katalizirana lakazom", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:131538

Metadata
TitleBiotransformacija katehola u mikroreaktoru katalizirana lakazom
Title (english)Laccase catalysed catechol biotransformation in a microreactor
AuthorIrena Đukić
MentorŽelimir Kurtanjek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Process Engineering)
(Laboratory for Measurement, Regulation and Automatisation)
Zagreb
Defense date and country2012-07-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Lakaze su enzimi iz skupine oksidoreduktaza koje imaju sposobnost biotransformacije fenolnih spojeve. U ovome radu provedena je biotransformacija katehola lakazom porijeklom iz Trametes versicolor u makro- i mikroreaktoru. Maksimalna konverzija postignuta u šaržnom makroreaktoru iznosila je X = 21,07 % nakon 1400 s, dok je konverzija od X = 16,73 % u mikroreaktoru s mješalima postignuta za vrijeme zadržavanja od 24 s. Prije provedbe šaržnog pokusa u makroreaktoru analizirana je stabilnost otopine katehola čuvane na T = 4 °C i na T = 25 °C čime je dokazana spontana degradacija katehola tijekom vremena. Također je uočeno smanjenje aktivnosti suspenzije lakaze čuvane na T = 4 °C tijekom vremena. Provedena je i analiza difuzivnosti katehola i lakaze u mikrorektoru. Korištenjem korelacijskih modela preuzetih iz literature izračunate su vrijednosti koeficijenata efektivne difuzivnosti za obje molekule. Rješavanjem matematičkog modela za difuziju u mikrorekatoru uočeno je da koeficijent difuzivnosti za katehol dobiven Reddy – Doraiswamy korelacijom daje najbolje slaganje s eksperimentalnim podacima za difuziju katehola u mikroreaktoru.
Abstract (english)
Laccases are enzymes belonging to group of oxidoreductase which have ability to perform biotransformation of phenolic compounds. In this work catechol biotransformation using laccase from Trametes versicolor was performed in macro- and microreactor. Performing experiments in macroscale batch reactor maximum conversion achieved was X = 21.07 % after 1400 s; reducing the reactor scale with the same initial conditions maximum conversion of X = 16.73 % was obtained in microreactor equipped with micromixers for residence time of 24 s. Before batch experiments stability of catechol solutions was analyzed. Catechol solutions were stored on two temperatures T = 4 °C and T = 25 °C. Spontaneous degradation of catechol with time was noticed. Also, decrease of laccase suspension activity on a temperature T = 4 °C was noticed. Diffusivity of catechol and laccase in microreactor was analysed. Using correlation models taken from literature, effective diffusivity coefficients for catechol and laccase were calculated. Performing simulations of mathematical model describing the diffusion in micoreactor it was noticed that diffusivity coefficient calculated using Reddy – Doraiswamy model describes the experimental data the most accurate.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:131538
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-09 07:43:08