prikaz prve stranice dokumenta Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura
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master's thesis
Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:658841
Dragosavljević, Jelena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Dragosavljević, J. (2012). Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:658841

Dragosavljević, Jelena. "Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:658841

Dragosavljević, Jelena. "Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:658841

Dragosavljević, J. (2012). 'Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:658841

Dragosavljević J. Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:658841

J. Dragosavljević, "Proizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:658841

Metadata
TitleProizvodnja i primjena autohtonih sojeva bakterija mliječne kiseline kao funkcionalnih starter kultura
Title (english)Production and application of autochthonous strains of lactic acid bacteria as functional starter cultures
AuthorJelena Dragosavljević
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2012-10-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom je radu provedeno ispitivanje sojeva bakterija mliječne kiseline L. lactis ZG5-9 i E. faecium ZG1-54 izoliranih iz autohtonog svježeg sira kao funkcionalnih starter kultura. Autohtoni izolati bakterija mliječne kiseline osjetljivi su na većinu ispitanih antibiotika, te su stoga sigurni s aspekta kontrole širenja antibiotičke rezistencije. Soj L. lactis ZG5-9 je osjetljiv na svih 12 ispitivanih antibiotika, a soj E. faecium ZG1-54 je rezistentan na streptomicin i neomicin. Oba bakterijska soja su pokazala dobro preživljavanje u simuliranim uvjetima gastrointestinalnog sustava. Rast odabranih sojeva E. faecium ZG1-54 i L. lactis ZG5-9, promjena pH i sinteza mliječne kiseline najveći su prilikom uzgoja u hranjivim podlogama s manitolom i laktulozom kao prebiotičkim supstratima. Stanice E. faecium ZG1-54 i L. lactis ZG5-9 pokazale su hidrofilna svojstva. Rezultati ispitivanja mikrobne adhezije na kloroform pokazuju afinitet ispitivanih BMK za to kiselo otapalo, što upućuje na njihova elektron-donorska (lužnata) svojstva. Bakterije E. faecium ZG1-54 i L. lactis ZG5-9 asimiliraju kolesterol u prisutnosti žučnih soli. Odabrani sojevi kao i soj Lactobacillus pentosus ZG225 su upotrijebljeni za proizvodnju svježeg sira kontroliranom fermentacijom. Masa sira proizvedenih s probiotičkim autohtonim sojevima Lactococcus lactis ZG5-9, Lactobacillus pentosus ZG2-25 i Enterococcus faecium ZG1-54 i sira proizvedenog s komercijalnom starter kulturom LL-50Y kao i prinos, pH vrijednost i postotak sinereze proizvedenih sireva se nisu značajno razlikovale.
Abstract (english)
The aim of this paper was investigation of lactic acid bacteria strains L. lactis ZG5-9 and E. faecium ZG1-54, isolated from the autochthonous fresh cheese, as functional starter cultures. Autochthonous lactic acid bacteria isolates were susceptible to most antibiotics tested, and are therefore safe from the standpoint of controlling the spread of antibiotic resistance. Strain L. lactis ZG5-9 is sensitive to all 12 tested antibiotics, a strain of E. faecium ZG1-54 is resistant to streptomycin and neomycin. Both bacterial strains showed good survival in simulated gastrointestinal system. Growth of selected strains of E. faecium ZG1-54 and L. lactis ZG5-9, a change in pH and lactic acid synthesis were highest during cultivation in nutrient media with mannitol and lactulose as prebiotic substrates. The cells of E. faecium ZG1-54 and L. lactis ZG5-9 showed hydrophilic properties. Test results of microbial adhesion to chloroform show an affinity for the tested LAB to acid solvent, which leads to their electron-donor (alkaline) performance. Bacteria E. faecium ZG1-54 and L. lactis ZG5-9 assimilate cholesterol in the presence of bile salts. Those strains and strain Lactobacillus pentosus ZG2-25 were used as functional starter cultures for fresh cheese production with controlled fermentation. Mass, yield, pH value and percentage of syneresis of probiotic cheese producted with autochthonous strains of Lactococcus lactis ZG5-9, Lactobacillus pentosus ZG2-25 and Enterococcus faecium ZG1-54 and the cheese produced with commercial starter culture LL-50Y were not significantly different.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:658841
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-09 08:14:44