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undergraduate thesis
Analiza DNA fragmenata metodom agarozne gel elektroforeze
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2023. urn:nbn:hr:151:939513
Mak, Maria Karla
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

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Mak, M. K. (2023). Analiza DNA fragmenata metodom agarozne gel elektroforeze (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:939513

Mak, Maria Karla. "Analiza DNA fragmenata metodom agarozne gel elektroforeze." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:151:939513

Mak, Maria Karla. "Analiza DNA fragmenata metodom agarozne gel elektroforeze." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:151:939513

Mak, M. K. (2023). 'Analiza DNA fragmenata metodom agarozne gel elektroforeze', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 14 November 2024, https://urn.nsk.hr/urn:nbn:hr:151:939513

Mak MK. Analiza DNA fragmenata metodom agarozne gel elektroforeze [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2023 [cited 2024 November 14] Available at: https://urn.nsk.hr/urn:nbn:hr:151:939513

M. K. Mak, "Analiza DNA fragmenata metodom agarozne gel elektroforeze", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:151:939513

Metadata
TitleAnaliza DNA fragmenata metodom agarozne gel elektroforeze
Title (english)DNA fragment analysis by agarose gel electrofhoresis method
AuthorMaria Karla Mak
MentorSonja Petrović (mentor)
Committee memberSonja Vila (član povjerenstva)
Committee memberSunčica Kujundžić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2023-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Agarozna gel elektroforeza je migracija nabijenih čestica na gelu, uslijed djelovanja električnog polja. Uobičajena je laboratorijska tehnika za odvajanje i analizu fragmenata DNA i ostalih biomolekula na temelju njihove veličine. Genomska DNA je ukupan genetički materijal jednog organizma i nalazi se u jezgrama stanica. Cilj ovog rada bio je opisati i primijeniti metodu agarozne gel elektroforeze te utvrditi veličine fragmenata DNA molekule. Pripremljene su četiri vrste gelova koncentracija: 0,8%, 1%, 2% i 3%. Prema dobivenim rezultatima utvrđeno je da su fragmenti genomske DNA veći od PCR fragmenata i sukladno s tim je zaključeno da manji fragmenti putuju brže i dalje od velikih fragmenata, što je bilo i očekivano. Zaključno, za analizu većih fragmenata genomske DNA optimalno je koristiti gel koncentracije 1% i manje, dok se za PCR fragmente najčešće koristi gel kocentracije od 2 do 3%, optimalno 2,5%.
Abstract (english)
Agarose gel electrophoresis is the migration of charged particles on the gel, due to the influence of applied electric field. It is a common laboratory technique for separating and analyzing DNA fragments and other biomolecules based on their size. Genomic DNA is a total genetic material of an organism and it's found in the cell nuclei. The aim of this research was to describe and apply the agarose gel electrophoresis method and to determine the sizes of DNA molecule fragments. Four gel concentrations were prepared: 0.8%, 1%, 2% and 3%. According to the obtained results, it was determined that genomic DNA fragments are larger than PCR fragments and accordingly it was concluded that smaller fragments travel faster than the large fragments, which was expected. In conclusion, for the analysis of larger fragments of genomic DNA, it is optimal to use a gel concentration of 1% or less, while for PCR fragments, gel concentration of 2 to 3% is usually used, optimally 2,5%.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:939513
Study programmeTitle: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture
Course: Plant production
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
File originBorn digital
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Created on2023-10-05 11:50:58