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Scientific paper - Original scientific paper
Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases
Acta Pharmaceutica, 69 (2019), 4; 649-660. https://doi.org/10.2478/acph-2020-0046
Brusač, Edvin; Jeličić, Mario-Livio; Amidžić Klarić, Daniela; Mornar, Ana

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Brusač, E., Jeličić, M. L., Amidžić Klarić, D. & Mornar, A. (2019). Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases. Acta Pharmaceutica, 69. (4), 649-660. doi: 10.2478/acph-2020-0046

Brusač, Edvin, et al. "Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases." Acta Pharmaceutica, vol. 69, no. 4, 2019, pp. 649-660. https://doi.org/10.2478/acph-2020-0046

Brusač, Edvin, Mario Livio Jeličić, Daniela Amidžić Klarić and Ana Mornar. "Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases." Acta Pharmaceutica 69, no. 4 (2019): 649-660. https://doi.org/10.2478/acph-2020-0046

Brusač, E., et al. (2019) 'Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases', Acta Pharmaceutica, 69(4), pp. 649-660. doi: 10.2478/acph-2020-0046

Brusač E, Jeličić ML, Amidžić Klarić D, Mornar A. Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases. Acta Pharmaceutica [Internet]. 2019 [cited 2024 December 24];69(4):649-660. doi: 10.2478/acph-2020-0046

E. Brusač, M. L. Jeličić, D. Amidžić Klarić and A. Mornar, "Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases", Acta Pharmaceutica, vol. 69, no. 4, pp. 649-660, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:163:979872. [Accessed: 24 December 2024]

Metadata
Title (english)Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases
AuthorEdvin Brusač
AuthorMario-Livio Jeličić
AuthorDaniela Amidžić Klarić
AuthorAna Mornar
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical analysis)
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medicinal chemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract (english)
A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was developed. Special attention was made to the most commonly reported problems in the measurement of distribution coefficients using a shake-flask method such as mixing technique, speed and time, the temperature of experiment, type of buffer and its pH as well as n-octanol/buffer phase ratio. The concentration of compounds in the buffer is determined by HPLC directly from shake flasks or conventional 2-mL vials. The developed method was fully validated according to ICH guidelines. Furthermore, experimental data were successfully compared with lipophilicity and human intestinal absorption calculated by the use of four different theoretical approaches. The method shows potential for high-throughput measurements of a large number of compounds.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleActa Pharmaceutica
Numberingvol. 69, no. 4, pp. 649-660
p-ISSN1330-0075
e-ISSN1846-9558
DOIhttps://doi.org/10.2478/acph-2020-0046
URN:NBNurn:nbn:hr:163:979872
Publication2019
Document URLhttps://hrcak.srce.hr/220976
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-04-07 15:06:09