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Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases
Acta Pharmaceutica, 69 (2019), 4; 649-660. https://doi.org/10.2478/acph-2020-0046
Brusač, Edvin; Jeličić, Mario-Livio; Amidžić Klarić, Daniela; Mornar, Ana

Institucijski repozitorij: Repozitorij Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu

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Brusač, E., Jeličić, M. L., Amidžić Klarić, D. i Mornar, A. (2019). Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases. Acta Pharmaceutica, 69. (4), 649-660. doi: 10.2478/acph-2020-0046

Brusač, Edvin, et al. "Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases." Acta Pharmaceutica, vol. 69, br. 4, 2019, str. 649-660. https://doi.org/10.2478/acph-2020-0046

Brusač, Edvin, Mario Livio Jeličić, Daniela Amidžić Klarić i Ana Mornar. "Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases." Acta Pharmaceutica 69, br. 4 (2019): 649-660. https://doi.org/10.2478/acph-2020-0046

Brusač, E., et al. (2019) 'Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases', Acta Pharmaceutica, 69(4), str. 649-660. doi: 10.2478/acph-2020-0046

Brusač E, Jeličić ML, Amidžić Klarić D, Mornar A. Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases. Acta Pharmaceutica [Internet]. 2019. [pristupljeno 25.12.2024.];69(4):649-660. doi: 10.2478/acph-2020-0046

E. Brusač, M. L. Jeličić, D. Amidžić Klarić i A. Mornar, "Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases", Acta Pharmaceutica, vol. 69, br. 4, str. 649-660, 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:163:979872. [Citirano: 25.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:163:979872
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Naslov (engleski)Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases
AutorEdvin Brusač
AutorMario-Livio Jeličić
AutorDaniela Amidžić Klarić
AutorAna Mornar
Autorova ustanovaSveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
(Zavod za analitiku i kontrolu lijekova)
Autorova ustanovaSveučilište u Zagrebu
Farmaceutsko-biokemijski fakultet
(Zavod za farmaceutsku kemiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Farmacija
Farmacija
Sažetak (engleski)
A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was developed. Special attention was made to the most commonly reported problems in the measurement of distribution coefficients using a shake-flask method such as mixing technique, speed and time, the temperature of experiment, type of buffer and its pH as well as n-octanol/buffer phase ratio. The concentration of compounds in the buffer is determined by HPLC directly from shake flasks or conventional 2-mL vials. The developed method was fully validated according to ICH guidelines. Furthermore, experimental data were successfully compared with lipophilicity and human intestinal absorption calculated by the use of four different theoretical approaches. The method shows potential for high-throughput measurements of a large number of compounds.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaActa Pharmaceutica
Brojčani podacivol. 69, br. 4, str. 649-660
p-ISSN1330-0075
e-ISSN1846-9558
DOIhttps://doi.org/10.2478/acph-2020-0046
URN:NBNurn:nbn:hr:163:979872
Datum objave publikacije2019
URL dokumentahttps://hrcak.srce.hr/220976
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2020-04-07 15:06:09