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master's thesis
Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:863319
Šitum, Magdalena
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical analysis

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Šitum, M. (2019). Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:863319

Šitum, Magdalena. "Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:863319

Šitum, Magdalena. "Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:863319

Šitum, M. (2019). 'Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:863319

Šitum M. Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:163:863319

M. Šitum, "Identifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:863319

Metadata
TitleIdentifikacija fitoestrogena u dodacima prehrani masenom spektrometrijom
Title (english)Identification of phytoestrogens in dietary supplements by mass spectrometry
AuthorMagdalena Šitum
MentorAna Mornar Turk (mentor)
Committee memberAna Mornar Turk (predsjednik povjerenstva)
Committee memberBiljana Nigović (član povjerenstva)
Committee memberŽivka Juričić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical analysis)
Zagreb
Defense date and country2019-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Fitoestrogeni su nesteroidni polifenolni spojevi koji se zbog svog djelovanja sličnog estrogenu sve češće koriste za otklanjanje nelagodnih simptoma menopauze kao sigurnija alternativa hormonskoj nadomjesnoj terapiji. Prirodni su im izvor različite biljke, poput soje, crvene djeteline, prstaste konopljike, lana i hmeljate su njihovi ekstrakti glavni sastojci brojnih dodataka prehrani prisutnih na tržištu. Upravo zbog široke dostupnosti i sve češće primjene dodataka prehrani, a upitne djelotvornosti i nedovoljne kontrole sastava potrebna je analitička metoda koja bi bila dovoljno brza, financijski isplativa, te primjenjiva u rutini kako bi se postigla poželjna sigurnost i kvaliteta ovakvih pripravaka. Cilj ovog istraživanja bio je razviti metodu identifikacije fitoestrogena direktnom injektiranjem na masenom spektrometru s ionizacijom elektroraspršenjem, koja bi omogućila identifikaciju svih fitoestrogena u takvim, višekomponentnim, pripravcima bez prethodnog odjeljivanja sastavnicama kromatografskim metodama. Metoda je uspješno primjenjena na analiziranim uzorcima, te bi kao takva mogla biti korisna kako proizvođačima, tako i regulatornim agencijama. Najveći nedostatak ove metode njena je ograničenost na kvalitativnu analizu.
Abstract (english)
Phytoestrogens are non-steroidal polyphenolic chemical entities that are often use for treatment of menopausal symptoms due to their estrogen-like activity as a safer alterative to hormone therapy. Their natural source are plants like soy, red clover, chaste tree, hop and flax and their extracts are main ingredients of large number of dietary supplements found on market. Because they are easily accessible and more often use than ever, even though their efficiency hasn´t been confirmed and their composition is insufficiently controlled, it is important to develop analytical method fast enough, profitable and also applicable on every day basis in order to achieve appropriate control of ingredients and quality. The aim of this research was to develop method of identification od phytoestrogens using direct injection electrospray ionization – mass spectrometry. The given method would allow identification of phytoestrogens in multicomponent formulations without previous separation with chromatographic methods. This method showed applicable for samples of dietary supplements and it can be of a significant meaning to both manufacturers and regulatory agencies. The biggest disadvantage of this method is its limitation to qualitative analysis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:863319
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-04-19 13:04:19