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master's thesis
Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:322275
Vičić, Valentina
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of analytical chemistry

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Vičić, V. (2021). Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:322275

Vičić, Valentina. "Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:322275

Vičić, Valentina. "Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:322275

Vičić, V. (2021). 'Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:163:322275

Vičić V. Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:163:322275

V. Vičić, "Optimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:322275

Metadata
TitleOptimizacija metode određivanja željeza u medu atomskom apsorpcijskom spektrometrijom
Title (english)Method optimization for determination of iron in honey by atomic absorption spectrophotometry
AuthorValentina Vičić
MentorJasna Jablan (mentor)
Committee memberJasna Jablan (predsjednik povjerenstva)
Committee memberŽivka Juričić (član povjerenstva)
Committee memberMaja Bival Štefan (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of analytical chemistry)
Zagreb
Defense date and country2021-06-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Željezo je jedan od najvažnijih elemenata za ljudski organizam. Njegove biološke uloge u tijelu čovjeka su mnogobrojne. Sastavni je dio velikog broja proteina, prostetskih skupina i kofaktor mnogim enzimima. Deficit željeza je najčešći deficit minerala u globalnoj populaciji, a pogađa posebno osjetljive skupine poput djece, adolescenata i mladih žena. Med je namirnica prirodnog porijekla bogatih nutritivnih svojstva i poznata po terapeutskim svojstvima. Kompleksni matriks oligosaharida čini ga zahtjevnim uzorkom za analizu vitamina i minerala. U ovom radu je pomoću Box-Behnkenovog dizajna optimizirana metoda pripreme uzorka za analizu sadržaja željeza u medu atomskom apsorpcijskom spektrometrijom. Višestrukom regresijskom analizom dobivena je kvadratna polinomna jednadžba koja opisuje utjecaj triju odabranih parametra (temperatura, udio kiseline i vrijeme) na sadržaj željeza u medu. Utvrđeni su optimalni parametri te je provedena analiza ukupno 21 različitih uzoraka meda. Ispitivanjem validacijskih parametara utvrđeno je da primijenjena metoda ima zadovoljavajuću linearnost, preciznost i točnost. Nakon analize uzoraka meda utvrđeno je da su koncentracije željeza u medu u korelaciji s rezultatima iz prethodnih istraživanja te da nema statistički značajne razlike između meda različitog geografskog porijekla.
Abstract (english)
Iron is one of the most important elements for the human body. Its biological roles in the human body are numerous. Iron is an integral part of many proteins, prosthetic groups, and a cofactor for many enzymes. Iron deficiency is the most common mineral deficiency in the global population, and particularly affects vulnerable groups such as children, adolescents, and young women. Honey is a food of natural origin with rich nutritional value and known for its therapeutic properties. The complex matrix of oligosaccharides makes it a challenging sample for vitamin and mineral analysis. In this work, the method of sample preparation was optimized for the analysis of iron content in honey by atomic absorption spectrophotometry using Box-Behnken design. Multiple regression analysis yielded a quadratic polynomial equation describing the influence of three selected parameters (temperature, acid content and time) on the iron content in honey. Optimal parameters were determined and a total of 21 different honey samples were analyzed. Validation of the analytical method showed that the method has satisfactory linearity, precision, and accuracy. After the analysis of honey samples, it was found that the iron concentrations in honey correlated with the results of previous studies and there was no statistically significant difference between honeys of different geographical origin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:322275
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-02-04 09:41:49