prikaz prve stranice dokumenta Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.
  Download
PDF 2.41 MB
master's thesis
Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. urn:nbn:hr:163:121402
Škugor, Doris
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmacognosy

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

Cite this document

Škugor, D. (2020). Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L. (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:121402

Škugor, Doris. "Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:121402

Škugor, Doris. "Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2020. https://urn.nsk.hr/urn:nbn:hr:163:121402

Škugor, D. (2020). 'Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:163:121402

Škugor D. Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2020 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:163:121402

D. Škugor, "Određivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:163:121402

Metadata
TitleOdređivanje antioksidacijskog djelovanja odabranih vrsta roda Salvia L.
Title (english)Determination of antioxidant activities of selected Salvia species
AuthorDoris Škugor
MentorSanda Vladimir-Knežević (mentor)
Committee memberSanda Vladimir-Knežević (predsjednik povjerenstva)
Committee memberMaja Bival Štefan (član povjerenstva)
Committee memberRenata Jurišić Grubešić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmacognosy)
Zagreb
Defense date and country2020-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
U okviru ovog diplomskog rada istraženi su prisutnost i antioksidacijski učinak fenolnih spojeva u metanolnim i etanolnim ekstraktima listova odabranih vrsta roda Salvia s područja Hrvatske (S. brachyodon, S. fruticosa, S.glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea i S. verticillata). Primjenom metode tankoslojne kromatografije dokazana je prisutnost ružmarinske kiseline i luteolin-7-O-glukozida u listovima svih uzoraka. Kvercitrin je prisutan samo u vrsti S. verticillata, a rutin u vrstama S. brachyodon, S. fruticosa i S. officinalis. Antioksidacijski učinak etanolnih ekstrakata ispitan je pomoću tri spektrofotometrijske metode u usporedbi s troloksom i EDTA. Utvrđena je sposobnost hvatanja DPPH radikala (IC50: 3,88-7,93 μg/mL), redukcije iona željeza(III) ( IC50: 9,43- 18,36 μg/mL) te keliranja iona željeza(II) ( IC50: 163,55-1592,53μg/mL). Dobiveni rezultati pokazuju da istraživane biljne vrste sadrže flavonoide i fenolne kiseline koji doprinose njihovim antioksidacijskim učincima.
Abstract (english)
The presence of phenolic compounds and their antioxidative effect in methanolic and ethanolic leaf extracts of selected species of the Salvia genus from Croatia (S. brachyodon, S. fruticosa, S.glutinosa, S. nemorosa, S. officinalis, S. pratensis, S. sclarea and S. verticillata) were studied in this diploma thesis. The presence of rosmarinic acid and luteolin-7-O-glucoside was detected by thin-layer chromatography in all leaf samples. Quercitrin was present only in the S. verticillata extract and rutin was detected in leaf extracts of S. brachyodon, S. fruticosa and S. officinalis. The antioxidative effects of ethanolic extracts were determined by using three spectrophotometric methods in comparison to trolox and EDTA. The abilities of scavenging DPPH radical (IC50: 3.88- 7.93 μg/mL), reduce ferric ions (IC50: 9.43-18.36 μg/mL) and chelate ferrous ions were determined. The obtained results have shown that the studied plant species contain flavonoids and phenolic acids which contribute to their antioxidative effects.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:121402
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-29
Terms of useLicence In copyright icon
Created on2022-08-26 13:18:58