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master's thesis
Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. urn:nbn:hr:163:774936
Novosel, Lucija
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Institutional repository: Repository of Faculty of Pharmacy and Biochemistry University of Zagreb

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Novosel, L. (2022). Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:774936

Novosel, Lucija. "Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:774936

Novosel, Lucija. "Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2022. https://urn.nsk.hr/urn:nbn:hr:163:774936

Novosel, L. (2022). 'Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:163:774936

Novosel L. Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2022 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:163:774936

L. Novosel, "Utvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:163:774936

Metadata
TitleUtvrđivanje delecije H69/V70 u SARS-CoV-2 virusu upotrebom TaqPath reagensa
Title (english)Determination of H69/V70 deletion in SARS-CoV-2 virus using TaqPath reagents
AuthorLucija Novosel
MentorMarija Grdić Rajković (mentor)
MentorIvana Ćelap (mentor)
Committee memberMarija Grdić Rajković (predsjednik povjerenstva)
Committee memberIvana Ćelap (član povjerenstva)
Committee memberDubravka Vitali Čepo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of medical biochemistry and haematology)
Zagreb
Defense date and country2022-11-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Virus SARS-CoV-2 velikom se brzinom proširio cijelim svijetom 2020. godine te uzrokovao globalnu pandemiju. Podložan je brojnim mutacijama koje na različite načine utječu na njegova svojstva kao i na rezultate molekularne analize RT-PCR kojom se potvrđuje infekcija ovim virusom. Cilj ovog diplomskog rada bio je usporediti rezultate RT-PCR testiranja uzoraka nazofaringealnih briseva na prisutnost virusa SARS-CoV-2 korištenjem komercijalnog test paketa koji detektira prisutnost više virusnih gena (ORF1ab, N i S protein) s rezultatima sekvenciranja različitih uzoraka kod kojih je RT-PCR metodom dokazana i kod kojih nije dokazana prisutnost S gena. Za provođenje testiranja korišten je TaqPathTM COVID-19 CE-IVD RT-PCR Kit, a sekvenciranje je provedeno NGS metodom u laboratoriju Eurofins Genomics Europe. Rezultati su potvrdili postojanje delecije H69/V70 kod svih uzoraka kod kojih RT-PCR metodom nije detektiran S gen. Utvrđeno je da postojanje delecije H69/V70 na S genu onemogućuje njegovu detekciju. S obzirom na to da se određuje prisutnost druga dva gena, lažno negativni rezultati za S gen ne znače odsustvo virusa nego ukazuju na potencijalno prisustvo ove mutacije u soju virusa koji kruži u populaciji.
Abstract (english)
The SARS-CoV-2 virus spread rapidly throughout the world and caused a global pandemic in 2020. The virus is susceptible to numerous mutations that affect to its properties in different ways. Mutations also affect to the results of the RT-PCR molecular analysis which is used to confirm virus infection. The aim of this study was to compare RT-PCR results of nasopharyngeal swab samples which were analyzed to detect the presence of the SARS-CoV-2 virus using a commercial test package that detects the presence of several viral genes (ORF1ab, N and S protein) with different sequencing results of samples where the presence of S protein was confirmed and samples where the presence of S protein was not confirmed using RT-PCR. The TaqPathTM COVID-19 CE-IVD RT-PCR Kit was used for testing and the sequencing was performed using the NGS method in the Eurofins Genomics Europe laboratory. The results confirmed deletion H69/V70 in every sample where the S gene was not detected using RT-PCR It was found that H69/V70 S gene deletion prevents S gene to be detected while PCR testing. False-negative results for S gene presence should not indicate the absence of the virus because there are two other gens which presence is detected. But negative S gene results can indicate the potential presence of this virus mutation and its spreading through population.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:774936
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-29 11:29:49