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master's thesis
Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR
Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:714511
Tkalčević, Mateja
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Tkalčević, M. (2021). Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:714511

Tkalčević, Mateja. "Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:714511

Tkalčević, Mateja. "Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:714511

Tkalčević, M. (2021). 'Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:714511

Tkalčević M. Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:714511

M. Tkalčević, "Istraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:714511

Metadata
TitleIstraživanje interakcija klaritromicina i ribosoma izoliranog iz bakterije Escherichia coli spektroskopijom NMR
Title (english)Study of interactions between clarithromycin and ribosome isolated from Escherichia coli by NMR spectroscopy
AuthorMateja Tkalčević
MentorTomislav Jednačak (mentor)
Committee memberTomislav Jednačak (predsjednik povjerenstva)
Committee memberNikola Bregović (član povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
Committee memberIvana Biljan (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2021-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Klaritromicin je predstavnik druge generacije makrolidnih antibiotika, u koju spada i poznatiji azitromicin. Makrolidni antibiotici (makrolidi) se koriste u liječenju infekcija uzrokovanih Gram-pozitivnim i Gram-negativnim bakterijama. Vezanjem za veliku podjedinicu bakterijskog ribosoma makrolidi inhibiraju translaciju proteina čime ubijaju bakterijske stanice. Učestalom primjenom makrolida bakterije su razvile različite mehanizme otpornosti na njihovo djelovanje. Stoga je interes znanosti preusmjeren na razvoj novih makrolidnih antibiotika. Za uspješan dizajn novog makrolida važno je razumjeti njegove interakcije s bakterijskim ribosomom. Stoga je cilj ovog rada okarakterizirati strukturu i interakcije klaritromcina s ribosomom izoliranim iz bakterije Escherichia coli u otopini primjenom jedno- i dvodimenzijskih tehnika NMR. Tehnikama NOESY (engl. nuclear Overhauser effect spectroscopy) i ROESY (engl. rotating frame Overhauser effect spectroscopy) određena je konformacija klaritromicina u slobodnom stanju, a pomoću prijenosnog NOESY određena je konformacija vezanog klaritromicina. Nadalje, metodom STD (engl. saturation transfer difference) proučene su interakcije klaritromicina s ribosomom iz bakterije E. coli i određeni su vezni epitopi.
Abstract (english)
Clarithromycin is a representative of the second generation of macrolide antibiotics, which includes better-known azithromycin. Macrolides are used for treating infections caused by Gram-positive and Gram-negative bacteria. Binding of macrolides to a large subunit of the bacterial ribosome inhibits protein translation and kills bacterial cells. Due to a frequent use of macrolides, bacteria have developed different resistance mechanisms. For this reason, the interest of science has been redirected to the development of new macrolide antibiotics. For a successful design of a new macrolide, it is important to understand its interactions with the bacterial ribosome. Therefore, the purpose of this study is to characterize the structure and interactions of clarithromycin with ribosome isolated from Escherichia coli in solution using one- and two-dimensional NMR techniques. Conformational studies of clarithromycin in the free-state were carried out using NOESY and ROESY techniques, while the bound-state conformation was determined from trNOESY correlations. Furthermore, the interactions of clarithromycin with the E. coli ribosome were studied by STD method, which allowed the assessment of binding epitopes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:714511
ProjectNumber: IP-2018-01-8098
Title: Makrozoni, novi konjugati makrolidnih antibiotika: Dizajn, priprava i interakcije
Title: Macrozones, New Conjugates of Macrolide Antibiotics: Design, Synthesis and Interactions
Acronym: Makrozoni/Macrozones
Leader: Predrag Novak
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-10-22 12:19:15