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master's thesis
Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:248784
Hajnić, Matea
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Hajnić, M. (2012). Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:248784

Hajnić, Matea. "Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:248784

Hajnić, Matea. "Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:248784

Hajnić, M. (2012). 'Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:248784

Hajnić M. Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:248784

M. Hajnić, "Modeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:248784

Metadata
TitleModeliranje stabilnosti heksamernog i dimernog oblika purinske nukleozidne fosforilaze iz bakterije Escherichia coli
Title (english)Stability of the dimeric and hexameric form of purine nucleoside phosphorylase from Escherichia coli - a computational study
AuthorMatea Hajnić
MentorBranimir Bertoša (mentor)
Committee memberBranimir Bertoša (predsjednik povjerenstva)
Committee memberIta Gruić Sovulj (član povjerenstva)
Committee memberKristian Vlahoviček (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Purinska nukleozidna fosforilaza (PNPaza) sudjeluje u pomodnom metaboličkom putu purina (od engl. “salvage pathway”) te omogućuje stanici da iskoristi komponente metaboličkog otpada za ponovnu sintezu nukleotida. Biološki aktivni oblik PNPaze u bakteriji Escherichia coli je homoheksamer koji se može opisati kao trimer dimera povezanih nekovalentnim interakcijama između kojih nije dokazana kooperativnost. Koristeći metode računalne biofizike istražena je mogućnost dobivanja stabilnih i funkcionalnih dimera uvođenjem mutacija u homoheksamernu strukturu divljeg tipa. Mutacije su uvedene s ciljem kidanja heksamerne strukture i stvaranja stabilnih dimera u otopini kako bi se provjerilo predstavlja li dimer osnovnu katalitičku jedinicu. Na temelju rezultata molekulske dinamike identificiran je mutant s kombinacijom mutacija koja bi mogla dovesti do raspada homoheksamerne strukture PNPaze u stabilne dimerne podjedinice.
Abstract (english)
Purine nucleoside phosphorylase (PNP) is one of the key enzymes in purine salvage pathway which helps cells to recover nucleotides from degradation intermediates of nucleic acids. Biologically active form of PNP in Escherichia coli is a homohexamer, also seen as a trimer of dimers mutually connected with non-covalent bonds, and still with no cooperativity proven between them. In order to examine if a dimer is sufficient for catalysis, combinations of mutations were introduced in silico into hexameric structure of wild type PNP from E.coli. Mutations or combinations thereof were introduced to disrupt the homohexameric form and to obtain stable dimers in solution. Based on the results obtained from molecular dynamic simulations, a mutant with the most promising combination of mutations was identified. These specific mutations could potentially cause the breakdown of hexameric structure and result in the formation of stable dimers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:248784
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 07:58:38