prikaz prve stranice dokumenta Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom
Embargo period 2024-10-19
master's thesis
Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:153741
Munivrana, Jana
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Munivrana, J. (2022). Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:153741

Munivrana, Jana. "Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:153741

Munivrana, Jana. "Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:153741

Munivrana, J. (2022). 'Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:153741

Munivrana J. Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 May 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:153741

J. Munivrana, "Određivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:153741

Metadata
TitleOdređivanje temperature glavnog faznog prijelaza neutralnih lipida UV/Vis spektroskopijom
Title (english)Determination of the main phase transition temperature of neutral lipids by UV/Vis spectroscopy
AuthorJana Munivrana
MentorDanijela Bakarić (mentor)
Committee memberAdriana Kenđel (predsjednik povjerenstva)
Committee memberTajana Begović (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberTomislav Jednačak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2022-10-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Lipidi u vodenom mediju tvore liposome, sferične strukture u kojima se slažu u dvosloje. Faza, odnosno način slaganja lipida u dvosloje, funkcija je temperature. Glavni fazni prijelaz je prijelaz lipida iz faze gela u fazu fluida i opaža se pri temperaturi mekšanja Tm. Uz razlikovnu pretražnu kalorimetriju kao rutinsku tehniku određivanja Tm odnedavno se ističe i UV/Vis spektroskopija. U sklopu ovog rada pripremljene su suspenzije 1,2-dipalmitoil-sn-glicero-3- fosfokolina (DPPC), 1,2-dipalmitoil-sn-glicero-3-fosfoetanolamina (DPPE), 2-oleil-1- palmitoil-glicero-3-fosfoetanolamina (POPE) i sfingomijelina (SM) u acetatnom, fosfatnom i karbonatnom puferu (pH = 4,09; 7,01; 9,19). Razlikovnom pretražnom kalorimetrijom i temperaturno-ovisnom UV/Vis spektroskopijom određene su vrijednosti Tm lipida te je ispitan utjecaj pH medija na Tm. Pokazano je da je temperaturno-ovisna UV/Vis spektroskopija prikladna za određivanje Tm te da promjena pH najviše utječe na DPPE i POPE liposome.
Abstract (english)
Lipids form liposomes in which they arrange into bilayers. The transition of lipids from the gel to the fluid phase, namely the main phase transition, is observed at the melting temperature Tm. Alongside differential scanning calorimetry (DSC), a routine technique for Tm determination, UV/Vis spectroscopy recently stood out. As part of this thesis, suspensions of 1,2-dipalmitoylsn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 2-oleyl-1-palmitoyl-glycero-3-phosphoethanolamine (POPE) and sphingomyelin (SM) in three buffers (pH = 4.09; 7.01; 9.19) were prepared. Using DSC and temperaturedependent UV/Vis spectroscopy, Tm of lipids were determined and the influence of pH on Tm was examined. It was shown that temperature-dependent UV/Vis spectroscopy is suitable for determining Tm and that DPPE and POPE liposomes are affected by the change in the pH.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:153741
ProjectNumber: UIP-2020-02-7669
Title: Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima
Title: Model of demyelination on a molecular scale at physiological and pathological conditions
Acronym: DEMYMOLSCALE
Leader: Danijela Bakarić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: UIP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2024-10-19
Terms of useLicence In copyright icon
Created on2022-11-04 10:42:19