prikaz prve stranice dokumenta Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)
Embargo period 2025-01-24
doctoral thesis
Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:895020
Vuk, Tamara
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Vuk, T. (2023). Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.) (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:895020

Vuk, Tamara. "Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:895020

Vuk, Tamara. "Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:895020

Vuk, T. (2023). 'Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:895020

Vuk T. Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.) [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:895020

T. Vuk, "Uloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:895020

Metadata
TitleUloga proteina BPM1 u mehanizmu metilacije DNA de novo tijekom razvoja uročnjaka (Arabidopsis thaliana L.)
Title (english)The role of BPM1 protein in de novo DNA methylation mechanism during development of Arabidopsis thaliana L.
AuthorTamara Vuk
MentorDunja Leljak-Levanić (mentor)
Committee memberMirjana Pavlica (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberSnježana Mihaljević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-01-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Proteini BPM iz vrste Arabidopsis thaliana primarnu ulogu ostvaruju u putu proteasomalne razgradnje ovisne o ubikvitinu, u sklopu kojeg prepoznaju specifične supstrate za razgradnju. Prethodno je utvrđena interakcija proteina BPM1 s proteinima RDM1 i DMS3, komponentama metilacijskog mehanizma RdDM. Uloga proteina BPM1 u mehanizmu RdDM u ovom je radu ispitana primjenom kromatinske imunoprecipitacije u svrhu identifikacije novih ciljnih gena ovog mehanizma u čijoj regulaciji metilacije posreduje protein BPM1. Identificirani su geni FBW2, RKP i AGL14, a kao referentni odabran je gen CML41. Analizom metilacije ovih gena metodom bisulfitnog sekvenciranja i pirosekvenciranja u linijama s prekomjernom ekspresijom proteina od interesa (oeBPM1, oeDMS3), te linijama s narušenom funkcijom mehanizma RdDM (dms3-1, nrpe1-10), potvrđen je stimulativni učinak proteina BPM1 na aktivnost mehanizma, koji je najizraženiji tijekom embriogeneze, a sam mehanizam RdDM značajniji je za zigotnu nego za somatsku embrigenezu. Ovisno o poziciji, učinci metilacijskih događaja reflektiraju se na ekspresiju svih analiziranih gena.
Abstract (english)
Proteins BPM of Arabidopsis thaliana have a primary role in the ubiquitin-dependent proteasomal degradation pathway, where they recognize specific substrates for degradation. The interaction of BPM1 protein with RDM1 and DMS3, components of RdDM methylation mechanism, has previously been established. Here, the role of BPM1 protein in RdDM was analyzed using chromatin immunoprecipitation for identification of new RdDM targets whose methylation is mediated by BPM1 protein. Genes FBW2, RKP and AGL14 were identified as targets, and CML41 was chosen as a reference gene. Methylation analysis of these genes was conducted using bisulfite sequencing and pyrosequencing in lines with overexpression of BPM1 (oeBPM1) or DMS3 (oeDMS3), and lines with impaired RdDM function (dms3-1, nrpe1-10). The results confirmed a stimulative role of BPM1 protein in RdDM. This mechanism proved to be more significant in zygotic than somatic embyrogenesis. Depending on the position, methylation had a diverse effect on expression of analyzed genes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:895020
Promotion2023
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-01-24
Terms of useLicence In copyright icon
Created on2023-01-31 12:20:09