prikaz prve stranice dokumenta Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro
Embargo period 2025-05-02
master's thesis
Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:007743
Gulin, Silvija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Gulin, S. (2023). Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:007743

Gulin, Silvija. "Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:007743

Gulin, Silvija. "Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:007743

Gulin, S. (2023). 'Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:007743

Gulin S. Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:007743

S. Gulin, "Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:007743

Metadata
TitleUčinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro
Title (english)The effect of high temperature cultivation on the bioactivity of broccoli sprouts (Brassica oleracea convar. botrytis) extracts during in vitro model of human digestion
AuthorSilvija Gulin
MentorIvana Šola (mentor)
Committee memberIvana Šola (predsjednik povjerenstva)
Committee memberDario Hruševar (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-05-02, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Visoke temperature značajno mijenjaju sadržaj metabolita u biljkama, nutritivnu vrijednost te biološke učinke ekstrakata biljaka. Cilj ovog rada bio je istražiti učinak visoke temperature uzgoja (38°C dan, 33°C noć) na koncentraciju različitih skupina i pojedinačnih spojeva u klijancima brokule (Brassica oleracea convar. botrytis) prije i tijekom in vitro simulirane probave, kao i na njihov antioksidacijski i antiglikacijski, te potencijal inhibicije enzima αamilaze, α-glukozidaze i lipaze. Visoka temperatura uzgoja povećala je udio i biodostupnost ukupnih fenolnih spojeva, flavonoida, flavonola, fenolnih i hidroksicimetnih kiselina u klijancima brokule, no rezultati su varirali ovisno o fazama probave. Na pojedinačne fenolne spojeve visoka temperatura uzgoja djelovala je značajnim sniženjem udjela vitamina C, povećanjem ferulične kiseline te kvercetina, dok na udio kempferola nije utjecala. Antioksidacijski potencijal ekstrakata klijanaca brokule porastao je prije probave mjeren metodom ABTS, dok je metoda DPPH pokazala obrnuti učinak. Visoka temperatura uzgoja smanjila je sposobnost ekstrakata da inhibiraju aktivnost enzima α-amilaze, povećala sposobnost inhibicije aktivnostienzima α-glukozidaze i glikacije proteina, nonakon probave rezultati su varirali ovisno o fazi probave i metodi mjerenja. Zaključujem da visoka temperatura uzgoja znatno može pridonijeti kvaliteti prehrane klijancima kupusnjača, no potrebne su detaljnije analize.
Abstract (english)
High temperatures significantly change metabolites content in plants, nutritional value and biological effects of plant extracts. The aim of this study was to investigate the effect of high growing temperature (38°C day, 33°C night) on the concentration of different groups and individual compounds in broccoli seedlings (Brassica oleracea convar. botrytis) before and during in vitro simulated digestion, as well as on their antioxidant, antiglycation and inhibition potential of α-amylase, α-glucosidase and lipase enzymes. High cultivation temperature increased the proportion and bioavailability of total phenolic compounds, flavonoids, flavonols, phenolic and hydroxycinnamic acids in broccoli sprouts, but the results varied depending on the stages of digestion. The high growing temperature affected individual phenolic compounds by significantly reducing the content of vitamin C, increasing ferulic acid and quercetin, while the content of kaempferol was not affected. The antioxidant potential of broccoli sprout extracts increased before digestion measured by the ABTS method, while the DPPH method showed the opposite effect. High cultivation temperature reduced the ability of extracts to inhibit α-amylase enzyme activity, increased the ability to inhibit α-glucosidase enzyme activity and protein glycation, but after digestion the results varied depending on the digestion phase and measurement method. Therefore, high growing temperature can significantly contribute to the quality of cabbage seedlings, but more detailed analyzes are needed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:007743
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-05-02
Terms of useLicence In copyright icon
Created on2023-05-12 09:18:53