prikaz prve stranice dokumenta Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles
  Download
PDF 1.73 MB
Scientific paper - Original scientific paper
Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles
Biophysical Journal, 120 (2021), 17; 3641-3648. https://doi.org/10.1016/j.bpj.2021.07.024
Ivec, Arian; Trupinić, Monika; Tolić, Iva M.; Pavin, Nenad

Citations:
,

Institutional repository: Repository of the Faculty of Science

Cite this document

Ivec, A., Trupinić, M., Tolić, I. M. & Pavin, N. (2021). Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles. Biophysical Journal, 120. (17), 3641-3648. doi: 10.1016/j.bpj.2021.07.024

Ivec, Arian, et al. "Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles." Biophysical Journal, vol. 120, no. 17, 2021, pp. 3641-3648. https://doi.org/10.1016/j.bpj.2021.07.024

Ivec, Arian, Monika Trupinić, Iva M. Tolić and Nenad Pavin. "Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles." Biophysical Journal 120, no. 17 (2021): 3641-3648. https://doi.org/10.1016/j.bpj.2021.07.024

Ivec, A., et al. (2021) 'Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles', Biophysical Journal, 120(17), pp. 3641-3648. doi: 10.1016/j.bpj.2021.07.024

Ivec A, Trupinić M, Tolić IM, Pavin N. Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles. Biophysical Journal [Internet]. 2021 September [cited 2025 March 28];120(17):3641-3648. doi: 10.1016/j.bpj.2021.07.024

A. Ivec, M. Trupinić, I. M. Tolić and N. Pavin, "Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles", Biophysical Journal, vol. 120, no. 17, pp. 3641-3648, September 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:021477. [Accessed: 28 March 2025]

Metadata
Title (english)Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles
AuthorArian Ivec
AuthorMonika Trupinić
AuthorIva M. Tolić
AuthorNenad Pavin
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
The highly ordered spatial organization of microtubule bundles in the mitotic spindle is crucial for its proper functioning. The recent discovery of twisted shapes of microtubule bundles and spindle chirality suggests that the bundles extend along curved paths in three dimensions, rather than being confined to a plane. This, in turn, implies that rotational forces, i.e., torques, exist in the spindle in addition to the widely studied linear forces. However, studies of spindle architecture and... More forces are impeded by a lack of a robust method for the geometric quantification of microtubule bundles in the spindle. In this work, we describe a simple method for measuring and evaluating the shapes of microtubule bundles by characterizing them in terms of their curvature and twist. By using confocal microscopy, we obtain three-dimensional images of spindles, which allows us to trace the entire microtubule bundle. For each traced bundle, we first fit a plane and then fit a circle lying in that plane. With this robust method, we extract the curvature and twist, which represent the geometric information characteristic for each bundle. As the bundle shapes reflect the forces within them, this method is valuable for the understanding of forces that act on chromosomes during mitosis. Less
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBiophysical Journal
Numberingvol. 120, no. 17, pp. 3641-3648
p-ISSN0006-3495
DOIhttps://doi.org/10.1016/j.bpj.2021.07.024
URN:NBNurn:nbn:hr:217:021477
Publication2021-09
ProjectNumber: 855158
Title (english): Molecular origins of aneuploidies in healthy and diseased human tissues
Acronym: ANEUPLOIDY
Jurisdiction: eu
Funding stream: Europska komisija
ProjectNumber: 647077
Title (english): A new class of microtubules in the spindle exerting forces on kinetochores
Acronym: NewSpindleForce
Jurisdiction: eu
Funding stream: H2020
ProjectNumber: IP-2019-04-5967
Title (croatian): Ravnoteža sila i momenta sila u diobenom vretenu
Title (english): Balance of forces and torques in the mitotic spindle
Acronym: SpindleForceBalance
Leader: Nenad Pavin
Jurisdiction: Croatia
Funding stream: IP
ProjectNumber: PZS-2019-02-7653
Title (croatian): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Title (english): Mechanisms of microtubule bundle formation required for spindle maturation
Acronym: SpindleForm
Leader: Iva Tolić
Jurisdiction: Croatia
Funding stream: PZS
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0006349521006329
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Public noteCopyright 2021 Biophysical Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Submitted March 1, 2021, and accepted for publication July 27, 2021.
Created on2023-07-28 20:45:39
accessibility

closeAccessibilityrefresh

If you wish to permanently save changes, click on Save, if not - your settings will be reset when you restart the browser.