prikaz prve stranice dokumenta Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles
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Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles
Biophysical Journal, 120 (2021), 17; 3641-3648. https://doi.org/10.1016/j.bpj.2021.07.024
Ivec, Arian; Trupinić, Monika; Tolić, Iva M.; Pavin, Nenad

Institucijski repozitorij: Repozitorij Prirodoslovno-matematičkog fakulteta

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Ivec, A., Trupinić, M., Tolić, I. M. i Pavin, N. (2021). Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles. Biophysical Journal, 120. (17), 3641-3648. doi: 10.1016/j.bpj.2021.07.024

Ivec, Arian, et al. "Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles." Biophysical Journal, vol. 120, br. 17, 2021, str. 3641-3648. https://doi.org/10.1016/j.bpj.2021.07.024

Ivec, Arian, Monika Trupinić, Iva M. Tolić i Nenad Pavin. "Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles." Biophysical Journal 120, br. 17 (2021): 3641-3648. https://doi.org/10.1016/j.bpj.2021.07.024

Ivec, A., et al. (2021) 'Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles', Biophysical Journal, 120(17), str. 3641-3648. doi: 10.1016/j.bpj.2021.07.024

Ivec A, Trupinić M, Tolić IM, Pavin N. Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles. Biophysical Journal [Internet]. 09.2021. [pristupljeno 28.11.2024.];120(17):3641-3648. doi: 10.1016/j.bpj.2021.07.024

A. Ivec, M. Trupinić, I. M. Tolić i N. Pavin, "Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles", Biophysical Journal, vol. 120, br. 17, str. 3641-3648, Rujan 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:021477. [Citirano: 28.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:021477
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Naslov (engleski)Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles
AutorArian Ivec
AutorMonika Trupinić
AutorIva M. Tolić
AutorNenad Pavin
Autorova ustanovaSveučilište u Zagrebu
Prirodoslovno-matematički fakultet
(Fizički odsjek)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Fizika
Sažetak (engleski)
The highly ordered spatial organization of microtubule bundles in the mitotic spindle is crucial for its proper functioning. The recent discovery of twisted shapes of microtubule bundles and spindle chirality suggests that the bundles extend along curved paths in three dimensions, rather than being confined to a plane. This, in turn, implies that rotational forces, i.e., torques, exist in the spindle in addition to the widely studied linear forces. However, studies of spindle architecture and forces are impeded by a lack of a robust method for the geometric quantification of microtubule bundles in the spindle. In this work, we describe a simple method for measuring and evaluating the shapes of microtubule bundles by characterizing them in terms of their curvature and twist. By using confocal microscopy, we obtain three-dimensional images of spindles, which allows us to trace the entire microtubule bundle. For each traced bundle, we first fit a plane and then fit a circle lying in that plane. With this robust method, we extract the curvature and twist, which represent the geometric information characteristic for each bundle. As the bundle shapes reflect the forces within them, this method is valuable for the understanding of forces that act on chromosomes during mitosis.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiophysical Journal
Brojčani podacivol. 120, br. 17, str. 3641-3648
p-ISSN0006-3495
DOIhttps://doi.org/10.1016/j.bpj.2021.07.024
URN:NBNurn:nbn:hr:217:021477
Datum objave publikacije2021-09
ProjektŠifra: 855158
Naziv (engleski): Molecular origins of aneuploidies in healthy and diseased human tissues
Kratica: ANEUPLOIDY
Pravna nadležnost: eu
Financijer: ERC-2019-SyG
Linija financiranja: Europska komisija
ProjektŠifra: 647077
Naziv (engleski): A new class of microtubules in the spindle exerting forces on kinetochores
Kratica: NewSpindleForce
Pravna nadležnost: eu
Financijer: EC
Linija financiranja: H2020
ProjektŠifra: IP-2019-04-5967
Naziv (hrvatski): Ravnoteža sila i momenta sila u diobenom vretenu
Naziv (engleski): Balance of forces and torques in the mitotic spindle
Kratica: SpindleForceBalance
Voditelj: Nenad Pavin
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
ProjektŠifra: PZS-2019-02-7653
Naziv (hrvatski): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Naziv (engleski): Mechanisms of microtubule bundle formation required for spindle maturation
Kratica: SpindleForm
Voditelj: Iva Tolić
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: PZS
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S0006349521006329
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Javna napomenaCopyright 2021 Biophysical Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Submitted March 1, 2021, and accepted for publication July 27, 2021.
Datum i vrijeme pohrane2023-07-28 20:45:39