prikaz prve stranice dokumenta Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis
Embargo period 2025-09-27
master's thesis
Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:419515
Petrović, Ana-Marija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Petrović, A. M. (2023). Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:419515

Petrović, Ana Marija. "Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:419515

Petrović, Ana Marija. "Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:419515

Petrović, A. M. (2023). 'Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:419515

Petrović AM. Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:419515

A. M. Petrović, "Odabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:419515

Metadata
TitleOdabir i evaluacija kandidatnih referentnih gena za analizu genske ekspresije u mozgu gušterica roda Podarcis
Title (english)Selection and evaluation of candidate reference genes for gene expression analysis in the brain of Podarcis lizards
AuthorAna-Marija Petrović
MentorSofia Ana Blažević (mentor)
Committee memberSofia Ana Blažević (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberInga Urlić (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Agresivnost je oblik kompetitivnog ponašanja koji snažno utječe na odnose između jedinki i vrsta. Izraženo agresivno ponašanje je primijećeno u odnosima jedinki vrsta Podarcis sicula (primorska gušterica) i Podarcis melisellensis (krška gušterica) na otocima i kontinentalnim dijelovima jadranske obale, gdje agresivnija P. sicula potiskuje P. melisellensis i smanjuje njen areal. Poznata je uloga monoamina u inicijaciji agresivnog ponašanja, naročito dopamina, serotonina i noradrenalina. Razlike u njihovim koncentracijama su u osnovi brojnih ponašanja i odraz su diferencijalne ekspresije njihovih metaboličkih gena. Profiliranje ekspresije gena pomoću RT-qPCR je robusna tehnika koja se sve češće koristi u neuroetološkim istraživanjima. Preduvjet za RT-qPCR analize je određivanje i provjera optimalnih referentnih gena koji će služiti za normalizaciju dobivenih rezultata. Cilj ovog diplomskog rada je analizirati stabilnost ekspresije deset potencijalnih referentnih gena (ACTB, GAPDH, GUSB, HMBS, HPRT1, PGK1, SDHA, TBP, TFRC i YWHA) i odabrati pet za buduće analize genske ekspresije, kao i optimizirati temperature taljenja odabranih parova početnica i učinkovitost umnažanja ciljanih sekvenci. Kao najstabilniji referentni geni su se pokazali: PGK1, YWHAZ, HMBS, HPRT1 i GUSB.
Abstract (english)
Aggression is a form of competitive behavior that strongly affects relationships between individuals and species. Pronounced aggressive behavior was observed in the relationship between the species Podarcis sicula (coastal lizard) i Podarcis melisellensis (karst lizard) on the islands and continental parts of the Adriatic coast, where the more aggressive P. sicula suppresses P. melisellensis and reduces its areal. The role of monoamines in the initiation of aggressive behavior is already known; dopamine, serotonin and noradrenaline in particular. The difference in their amounts is the basis of numerous types of behaviors and is a reflection of the differential expression of their metabolic genes. Gene expression profiling using RT-qPCR is a robust technique that is increasingly being used in neuroethological research. A prerequisite for RT-qPCR analysis is the determination and validation of optimal reference genes that will serve to normalize the obtained data. The aim of this thesis is to analyze the expression stability of ten potential reference genes (ACTB, GAPDH, GUSB, HMBS, HPRT1, PGK1, SDHA, TBP, TFRC and YWHA) and to select five for future gene expression analysis, as well as to optimize the melting temperatures of the selected primer pairs and amplification efficiencies of target sequences. The most stably expressed reference genes were determined to be: PGK1, YWHAZ, HMBS, HPRT1 and GUSB.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:419515
ProjectNumber: UIP-2019-04-8469
Title: Dopaminska regulacija kompetitivnog ponašanja u kohabituirajućim populacijama guštera Podarcis siculus i Podarcis melisellensis
Title: Dopamine regulation of competitive behavior in coexisting populations of lizards Podarcis siculus and Podarcis melisellensis
Acronym: BOLDer
Leader: Sofia Ana Blažević
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: UIP
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-09-27
Terms of useLicence In copyright icon
Created on2023-10-17 11:17:57