prikaz prve stranice dokumenta Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama
Embargo period 2026-02-16
master's thesis
Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:576107
Otmačić, Lucija
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Otmačić, L. (2024). Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:576107

Otmačić, Lucija. "Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:576107

Otmačić, Lucija. "Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:576107

Otmačić, L. (2024). 'Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:576107

Otmačić L. Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:576107

L. Otmačić, "Termodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:576107

Metadata
TitleTermodinamička i strukturna karakterizacija kompleksa ciklopeptida s kolorimetrijskim i fluorescentnim anionskim bojama
Title (english)Thermodynamic and structural characterization of cyclopeptide complexes with colorimetric and fluorescent anionic dyes
AuthorLucija Otmačić
MentorGordan Horvat (mentor)
Committee memberGordan Horvat (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberAleksandra Maršavelski (član povjerenstva)
Committee memberVladislav Tomišić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2024-02-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom su radu ispitana spektroskopska i strukturna svojstva te termodinamička stabilnost kompleksa homociklopentapeptida s kolorimetrijskim i fluorescentnim anionskim bojama u acetonitrilu pomoću spektrofluorimetrijskih i spektrofotometrijskih titracija i simulacija molekulske dinamike. Kao ciklopeptidi korišteni su ciklopentafenilalanin, ciklopentaleucin i derivat ciklopentalizina čije su amino skupine na bočnim ograncima zaštićene BOC skupinama. Kao reprezentativni anioni sulfonatnih fluorescentnih boja korišteni su 8-anilino-1-naftalensulfonska kiselina i alizarin crveno S, a kao reprezentativni anion sulfonatnih kolorimetrijskih boja korišten je metiloranž. Kao reprezentativni anioni karboksilatnih fluorescentnih boja korištene su N-acetilirane aromatske amino kiseline triptofan i fenilalanin. Rezultati upućuju na stvaranje stabilnih kompleksa između ciklopepentaleucina i derivata ciklopentalizina sa sulfonatnim i karboksilatnim fluorescentnim i kolorimetrijskim bojama, dok je u slučaju ciklopentafenilalanina afinitet prema fluorescentnim karboksilatnim bojama nešto viši. Kod nastalih kompleksa ciklopeptida s fluorescentnim sulfonatnim i karboksilatnim bojama u većini slučajeva opažena je značajna razlika u molarnim spektrima kompleksa, dok ta razlika nije uočena kod kompleksa s kolorimetrijskom sulfonatnom bojom. Također, rezultati provedenih MD simulacija u otapalu upućuju na koordinaciju aniona preko amidnih protona ciklopeptida.
Abstract (english)
In this work, spectroscopic and structural properties, as well as thermodynamic stability of homocyclopentapeptide complexes with colorimetric and fluorescent anionic dyes in acetonitrile were examined by means of spectrofluorimetric and spectrophotometric titrations and molecular dynamics simulations. As cyclopeptides, cyclopentaphenylalanine, cyclopentaleucine and cyclopentalysine derivative with BOC protected amino group side chains were used. 8-anilino-1-naftalenesulfonic acid and alizarin red S were used as sulfonic fluorescent dyes representatives, while methyl orange was used as sulfonic colorimetric dye representative. N-acetylated aromatic amino acids tryptophan and phenylalanine were used as carboxylic fluorescent dyes representatives. The results indicate that both of cyclic peptides form stabile complexes with fluorescent and colorimetric sulfonic and carboxylic dyes, while the affinity of cyclopentaphenylalanine towards fluorescent carboxylic dyes is somewhat higher. In most cases a significant difference in molar spectra of fluorescent dye complexes with both peptides were observed. That significant difference was not observed with colorimetric anion dye complexes. Also, the results obtained by MD simulations indicate that the anions are coordinated by the amide protons of the peptide.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:576107
ProjectNumber: IP-2019-04-9560
Title: Koordinacijske reakcije makrocikličkih liganada u otopini
Title: Coordination Reactions of Macrocyclic Ligands in Solution
Acronym: MacroSol
Leader: Vladislav Tomišić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Graduate university studies in chemistry
Course: Graduate university studies in chemistry
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-02-16
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Created on2024-03-12 12:24:16