prikaz prve stranice dokumenta The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
  Download
PDF 6.6 MB
Scientific paper - Original scientific paper
The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
Current Biology, 32 (2022), 11; 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035
Trupinić, Monika; Kokanović, Barbara; Ponjavić, Ivana; Barišić, Ivan; Šegvić, Siniša; Ivec, Arian; Tolić, Iva M.

Institutional repository: Repository of the Faculty of Science

Cite this document

Trupinić, M., Kokanović, B., Ponjavić, I., Barišić, I., Šegvić, S., Ivec, A. & Tolić, I. M. (2022). The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology, 32. (11), 2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

Trupinić, Monika, et al. "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin." Current Biology, vol. 32, no. 11, 2022, pp. 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Trupinić, Monika, Barbara Kokanović, Ivana Ponjavić, Ivan Barišić, Siniša Šegvić, Arian Ivec and Iva M. Tolić. "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin." Current Biology 32, no. 11 (2022): 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Trupinić, M., et al. (2022) 'The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin', Current Biology, 32(11), pp. 2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

Trupinić M, Kokanović B, Ponjavić I, Barišić I, Šegvić S, Ivec A, and sur.. The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology [Internet]. 2022 June [cited 2024 August 25];32(11):2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

M. Trupinić, et al., "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin", Current Biology, vol. 32, no. 11, pp. 2480-2493.e6, June 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:003399. [Accessed: 25 August 2024]

Metadata
Title (english)The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
AuthorMonika Trupinić
AuthorBarbara Kokanović
AuthorIvana Ponjavić
AuthorIvan Barišić
AuthorSiniša Šegvić
AuthorArian Ivec
AuthorIva M. Tolić
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
Forces produced by motor proteins and microtubule dynamics within the mitotic spindle are crucial for proper chromosome segregation. In addition to linear forces, rotational forces or torques are present in the spindle, which are reflected in the left-handed twisted shapes of microtubule bundles that make the spindle chiral. However, the biological role and molecular origins of spindle chirality are unknown. By developing methods for measuring the spindle twist, we show that spindles are most chiral near the metaphase-to-anaphase transition. To assess the role of chirality in spindle mechanics, we compressed the spindles along their axis. This resulted in a stronger left-handed twist, suggesting that the twisted shape allows for a mechanical response to forces. Inhibition or depletion of motor proteins that perform chiral stepping, Eg5/kinesin-5, Kif18A/kinesin-8, MKLP1/kinesin-6, and dynein, decreased the left-handed twist or led to right- handed twist, implying that these motors regulate the twist by rotating microtubules within their antiparallel overlaps or at the spindle pole. A right-handed twist was also observed after the depletion of the microtubule nucleator augmin, indicating its contribution to the twist through the nucleation of antiparallel bridging microtubules. The uncovered switch from left- handed to right-handed twist reveals the existence of competing mechanisms that promote twisting in opposite directions. As round spindles are more twisted than the elongated ones are, we infer that bending and twisting moments are generated by similar molecular mechanisms and propose a physiological role for spindle chirality in allowing the spindle to absorb mechanical load.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCurrent Biology
Numberingvol. 32, no. 11, pp. 2480-2493.e6
p-ISSN0960-9822
DOIhttps://doi.org/10.1016/j.cub.2022.04.035
URN:NBNurn:nbn:hr:217:003399
Publication2022-06
ProjectNumber: PZS-2019-02-7653
Title (croatian): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Title (english): Mechanisms of microtubule bundle formation required for spindle maturation
Acronym: SpindleForm
Leader: Iva Marija Tolić
Jurisdiction: Croatia
Funding stream: Programme Research Cooperability
ProjectNumber: IP-2019-04-5967
Title (croatian): Ravnoteža sila i momenta sila u diobenom vretenu
Title (english): Balance of forces and torques in the mitotic spindle
Acronym: SpindleForceBalance
Leader: Nenad Pavin
Jurisdiction: Croatia
Funding stream: Research Projects
ProjectNumber: KK.01.1.1.04.0057
Title (croatian): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini
Title (english): Inovative microscopy protocols for interdisciplinary research in biomedicine
Acronym: IPSted
Leader: Iva Marija Tolić
Jurisdiction: eu
Funding stream: European Regional Development Fund
ProjectNumber: 855158
Title (croatian): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima
Title (english): Molecular origins of aneuploidies in healthy and diseased human tissues
Acronym: ANEUPLOIDY
Leader: Iva Marija Tolić
Jurisdiction: eu
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0960982222006042
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received: September 23, 2021. Received in revised form: February 17, 2022. Accepted: April 13, 2022. Published: May 9, 2022.
Created on2024-08-22 19:52:46