prikaz prve stranice dokumenta The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
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The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
Current Biology, 32 (2022), 11; 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035
Trupinić, Monika; Kokanović, Barbara; Ponjavić, Ivana; Barišić, Ivan; Šegvić, Siniša; Ivec, Arian; Tolić, Iva M.

Institucijski repozitorij: Repozitorij Prirodoslovno-matematičkog fakulteta

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Trupinić, M., Kokanović, B., Ponjavić, I., Barišić, I., Šegvić, S., Ivec, A. i Tolić, I. M. (2022). The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology, 32. (11), 2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

Trupinić, Monika, et al. "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin." Current Biology, vol. 32, br. 11, 2022, str. 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Trupinić, Monika, Barbara Kokanović, Ivana Ponjavić, Ivan Barišić, Siniša Šegvić, Arian Ivec i Iva M. Tolić. "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin." Current Biology 32, br. 11 (2022): 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Trupinić, M., et al. (2022) 'The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin', Current Biology, 32(11), str. 2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

Trupinić M, Kokanović B, Ponjavić I, Barišić I, Šegvić S, Ivec A, i sur.. The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology [Internet]. 06.2022. [pristupljeno 27.12.2024.];32(11):2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

M. Trupinić, et al., "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin", Current Biology, vol. 32, br. 11, str. 2480-2493.e6, Lipanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:003399. [Citirano: 27.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:003399
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Naslov (engleski)The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
AutorMonika Trupinić
AutorBarbara Kokanović
AutorIvana Ponjavić
AutorIvan Barišić
AutorSiniša Šegvić
AutorArian Ivec
AutorIva M. Tolić
Autorova ustanovaSveučilište u Zagrebu
Prirodoslovno-matematički fakultet
(Fizički odsjek)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Fizika
Sažetak (engleski)
Forces produced by motor proteins and microtubule dynamics within the mitotic spindle are crucial for proper chromosome segregation. In addition to linear forces, rotational forces or torques are present in the spindle, which are reflected in the left-handed twisted shapes of microtubule bundles that make the spindle chiral. However, the biological role and molecular origins of spindle chirality are unknown. By developing methods for measuring the spindle twist, we show that spindles are most chiral near the metaphase-to-anaphase transition. To assess the role of chirality in spindle mechanics, we compressed the spindles along their axis. This resulted in a stronger left-handed twist, suggesting that the twisted shape allows for a mechanical response to forces. Inhibition or depletion of motor proteins that perform chiral stepping, Eg5/kinesin-5, Kif18A/kinesin-8, MKLP1/kinesin-6, and dynein, decreased the left-handed twist or led to right- handed twist, implying that these motors regulate the twist by rotating microtubules within their antiparallel overlaps or at the spindle pole. A right-handed twist was also observed after the depletion of the microtubule nucleator augmin, indicating its contribution to the twist through the nucleation of antiparallel bridging microtubules. The uncovered switch from left- handed to right-handed twist reveals the existence of competing mechanisms that promote twisting in opposite directions. As round spindles are more twisted than the elongated ones are, we infer that bending and twisting moments are generated by similar molecular mechanisms and propose a physiological role for spindle chirality in allowing the spindle to absorb mechanical load.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCurrent Biology
Brojčani podacivol. 32, br. 11, str. 2480-2493.e6
p-ISSN0960-9822
DOIhttps://doi.org/10.1016/j.cub.2022.04.035
URN:NBNurn:nbn:hr:217:003399
Datum objave publikacije2022-06
ProjektŠifra: PZS-2019-02-7653
Naziv (hrvatski): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Naziv (engleski): Mechanisms of microtubule bundle formation required for spindle maturation
Kratica: SpindleForm
Voditelj: Iva Marija Tolić
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Programme Research Cooperability
ProjektŠifra: IP-2019-04-5967
Naziv (hrvatski): Ravnoteža sila i momenta sila u diobenom vretenu
Naziv (engleski): Balance of forces and torques in the mitotic spindle
Kratica: SpindleForceBalance
Voditelj: Nenad Pavin
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
ProjektŠifra: KK.01.1.1.04.0057
Naziv (hrvatski): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini
Naziv (engleski): Inovative microscopy protocols for interdisciplinary research in biomedicine
Kratica: IPSted
Voditelj: Iva Marija Tolić
Pravna nadležnost: eu
Financijer: Europska komisija
Linija financiranja: European Regional Development Fund
ProjektŠifra: 855158
Naziv (hrvatski): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima
Naziv (engleski): Molecular origins of aneuploidies in healthy and diseased human tissues
Kratica: ANEUPLOIDY
Voditelj: Iva Marija Tolić
Pravna nadležnost: eu
Financijer: Europska komisija
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S0960982222006042
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Javna napomena© 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received: September 23, 2021. Received in revised form: February 17, 2022. Accepted: April 13, 2022. Published: May 9, 2022.
Datum i vrijeme pohrane2024-08-22 19:52:46