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master's thesis
Identification and characterization of BPM2 splice variants in Arabidopsis thaliana
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:156156
Keresteš, Gaj
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Keresteš, G. (2024). Identification and characterization of BPM2 splice variants in Arabidopsis thaliana (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:156156

Keresteš, Gaj. "Identification and characterization of BPM2 splice variants in Arabidopsis thaliana." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:156156

Keresteš, Gaj. "Identification and characterization of BPM2 splice variants in Arabidopsis thaliana." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:156156

Keresteš, G. (2024). 'Identification and characterization of BPM2 splice variants in Arabidopsis thaliana', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:156156

Keresteš G. Identification and characterization of BPM2 splice variants in Arabidopsis thaliana [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:156156

G. Keresteš, "Identification and characterization of BPM2 splice variants in Arabidopsis thaliana", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:156156

Metadata
TitleIdentification and characterization of BPM2 splice variants in Arabidopsis thaliana
Title (croatian)Identifikacija i karakterizacija varijanti alternativnog prekrajanja uročnjakova gena BPM2
AuthorGaj Keresteš
MentorDunja Leljak-Levanić (mentor)
MentorPaula Štancl (komentor)
Committee memberDunja Leljak-Levanić (predsjednik povjerenstva)
Committee memberRosa Karlić (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
Committee memberMirjana Pavlica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
IMATH-BTB proteins mediate turnover of specific proteins by acting as substrate adaptors of E3 ubiquitin ligases. The MATH-BTB gene family has undergone a significant expansion in grasses, with genomes of different grass species having dozens of MATH-BTB genes, some of which have roles in fundamental developmental processes. In contrast, the genome of Arabidopsis thaliana only contains six MATH-BTB genes: BPM1-6. In this thesis, alternative splicing was explored as a potential mechanism for A. thaliana to achieve the number and diversity of MATH-BTB proteins similar to grasses. A bioinformatic analysis of the newest A. thaliana transcriptome, AtRTD3, revealed 56 transcript isoforms encoded by BPM genes, 16 of which are encoded by BPM2. While proteins encoded by the 16 BPM2 transcripts differed in domain composition, they lacked the diversity in MATH domains that's present in grass-specific MATH-BTB proteins. Expressions of splice variants BPM2.3, BPM2.8, BPM2.9 and BPM2.15 were analyzed in some vegetative, reproductive and embryonic tissues with standard and quantitative PCR. Splice variants BPM2.3 and BPM2.15 had significant differences in expression levels across different tissues, indicating a possible role in developmental transitions. Theoretically unexpected amplicons represent potentially novel splice variants.
Abstract (croatian)
Proteini MATH-BTB sudjeluju u prometu specifičnih proteina djelujući kao adapteri supstrata E3 ubikvitinskih ligaza. Porodica gena MATH-BTB je prošla značajnu ekspanziju u travama, pri čemu genomi različitih vrsta trava imaju desetke gena MATH-BTB, od kojih neki imaju uloge u temeljnim razvojnim procesima. Nasuprot tome, genom uročnjaka Arabidopsis thaliana ima samo šest MATH-BTB gena: BPM1-6. U ovom radu, alternativno prekrajanje transkripata je istraženo kao potencijalni mehanizam kojim A. thaliana postiže broj i raznolikost proteina MATH-BTB sličan travama. Bioinformatička analiza najnovijeg transkriptoma A. thaliana, AtRTD3, otkrila je 56 izoformi transkripta kodiranih genima BPM, od kojih je 16 kodirano genom BPM2. Iako su se proteini kodirani sa 16 transkripata gena BPM2 razlikovali u sadržaju domena, nedostajala im je raznolikost u MATH domenama koja je prisutna u MATH-BTB proteinima specifičnima za trave. Ekspresije varijanata prekrajanja BPM2.3, BPM2.8, BPM2.9 i BPM2.15 analizirane su u nekim vegetativnim, reproduktivnim i embrionalnim tkivima standardnim i kvantitativnim reakcijama PCR-a. Varijante BPM2.3 i BPM2.15 imale su značajne razlike u razinama ekspresije u različitim tkivima, što ukazuje na moguću ulogu u razvojnim prijelazima. Standardni PCR je proizveo teoretski neočekivane amplikone koji predstavljaju potencijalno nove varijante prekrajanja
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:156156
ProjectNumber: IP-2022-10-7874
Title: Alternativno prekrajanje gena BPM2 kao mehanizam uspostave funkcionalne raznolikosti porodice proteina MATH-BTB u uročnjaku Arabidopsis thaliana
Title: Alternative splicing of BPM2 as a mechanism for establishing a functional diversity of MATH-BTB protein family in Arabidopsis thaliana
Acronym: SpliceFun
Leader: Nataša Bauer
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Research Projects
Study programmeTitle: Molecular Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-08-26 12:51:31