prikaz prve stranice dokumenta Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly
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Scientific paper - Original scientific paper
Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly
Nature Communications, 13 (2022), 1; 7307. https://doi.org/10.1038/s41467-022-34957-4
Matković, Jurica; Ghosh, Subhadip; Ćosić, Mateja; Eibes, Susana; Barišić, Marin; Pavin, Nenad; Tolić, Iva M.

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Matković, J., Ghosh, S., Ćosić, M., Eibes, S., Barišić, M., Pavin, N. & Tolić, I. M. (2022). Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly. Nature Communications, 13. (1). doi: 10.1038/s41467-022-34957-4

Matković, Jurica, et al. "Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly." Nature Communications, vol. 13, no. 1, 2022. https://doi.org/10.1038/s41467-022-34957-4

Matković, Jurica, Subhadip Ghosh, Mateja Ćosić, Susana Eibes, Marin Barišić, Nenad Pavin and Iva M. Tolić. "Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly." Nature Communications 13, no. 1 (2022). https://doi.org/10.1038/s41467-022-34957-4

Matković, J., et al. (2022) 'Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly', Nature Communications, 13(1). doi: 10.1038/s41467-022-34957-4

Matković J, Ghosh S, Ćosić M, Eibes S, Barišić M, Pavin N, and sur.. Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly. Nature Communications [Internet]. 2022 November 27 [cited 2024 December 29];13(1). doi: 10.1038/s41467-022-34957-4

J. Matković, et al., "Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly", Nature Communications, vol. 13, no. 1, November 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:679274. [Accessed: 29 December 2024]

Metadata
Title (english)Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly
AuthorJurica Matković
AuthorSubhadip Ghosh
AuthorMateja Ćosić
AuthorSusana Eibes
AuthorMarin Barišić
AuthorNenad Pavin
AuthorIva M. Tolić
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
Mitotic spindle assembly is crucial for chromosome segregation and relies on bundles of microtubules that extend from the poles and overlap in the middle. However, how these structures form remains poorly understood. Here we show that overlap bundles arise through a network-to-bundles transition driven by kinetochores and chromosomes. STED super-resolution microscopy reveals that PRC1-crosslinked microtubules initially form loose arrays, which become rearranged into bundles. Kinetochores promote microtubule bundling by lateral binding via CENP-E/kinesin-7 in an Aurora B-regulated manner. Steric interactions between the bundle-associated chromosomes at the spindle midplane drive bundle separation and spindle widening. In agreement with experiments, theoretical modeling suggests that bundles arise through competing attractive and repulsive mechanisms. Finally, perturbation of overlap bundles leads to inefficient correction of erroneous kinetochore-microtubule attachments. Thus, kinetochores and chromosomes drive coarsening of a uniform microtubule array into overlap bundles, which promote not only spindle formation but also chromosome segregation fidelity.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleNature Communications
Numberingvol. 13, no. 1, 7307
e-ISSN2041-1723
DOIhttps://doi.org/10.1038/s41467-022-34957-4
URN:NBNurn:nbn:hr:217:679274
Publication2022-11-27
ProjectNumber: PZS-2019-02-7653
Title (croatian): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Title (english): Mechanisms of microtubule bundle formation required for spindle maturation
Acronym: SpindleForm
Leader: Iva Marija Tolić
Jurisdiction: Croatia
Funding stream: Programme Research Cooperability
ProjectNumber: 855158
Title (croatian): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima
Title (english): Molecular origins of aneuploidies in healthy and diseased human tissues
Acronym: ANEUPLOIDY
Leader: Iva Marija Tolić
Jurisdiction: eu
ProjectNumber: KK.01.1.1.04.0057
Title (croatian): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini
Title (english): Inovative microscopy protocols for interdisciplinary research in biomedicine
Acronym: IPSted
Leader: Iva Marija Tolić
Jurisdiction: eu
Funding stream: European Regional Development Fund
Document URLhttps://www.nature.com/articles/s41467-022-34957-4
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© The Author(s) 2022. s This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received: 28 March 2022; Accepted: 11 November 2022; Published: 27 November 2022.
Created on2024-08-31 20:53:40