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master's thesis
Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:353069
Burić, Duje
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Burić, D. (2009). Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:353069

Burić, Duje. "Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:353069

Burić, Duje. "Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:353069

Burić, D. (2009). 'Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:353069

Burić D. Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2025 January 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:353069

D. Burić, "Karakterizacija domene koja vezuje ubikvitin u proteinu C1orf124", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:353069

Metadata
TitleKarakterizacija domene koja vezuje ubikvitin u proteinu C1orf124
Title (english)Characterization of ubiquitin-binding domain in C1orf124 protein
AuthorDuje Burić
MentorIvan Đikić (mentor)
MentorMaja Matulić (sumentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Novija istraživanja pokazala su da ubikvitin ima ključnu ulogu u različitim biološkoim procesima. Također su otkrivene proteinske domene koje mogu vezivati različite ubikvitinske vrste. Bioinformatička analiza nedavno je otkrila obitelj domena koje vezuju ubikvitin sa specifičnom strukturom cinkovog prsta, tzv. domenu UBZ4. Sadrži cinkov prst C2HC i nalazimo je u proteinima eukariota svih linija. Za većinu proteina koji sadrže UBZ4 domenu vjeruje se da imaju bitnu ulogu u odgovoru na oštećenja DNA molekule u stanici. Iterativno sravnjivanje s poznatim UBZ4 otkrilo je 13 članova pretpostavljene obitelji. Protein C1orf124 je izabran za istraživanje njegovog vezanja na ubikvitin. Ovim istraživanjem pokazao sam da protein C1orf124 veže monoubkvitin i poliubikvitinske lance. Afinitetna kromatografija pokazala je da se interakcija postiže preko hidrofobne regije na površini ubikvitina koja vrlo konzerviran izoleucinski aminokiselinski ostatak na 44 mjestu i aspartatnog ostatka unutar druge dijade koja vezuje atom cinka domene UBZ4.
Abstract (english)
Ubiquitin has recently been discovered to have a key role in a variety of biological processes. In parallel, different protein domains were identified with the ability to bind different ubiquitin species. Ubiquitin-binding zinc-finger 4 (UBZ4) family was recently revealed using bioinformatical analysis. UBZ4 is a C2HC Zn-finger found in all eukaryotic lineages; most of the UBZ4-containing proteins appear to play a role in the DNA damage response. Iterative alignment with known UBZ4 revealed 13 members of putative family. C1orf124 protein was chosen to analyse its ubiquitin binding abilities. I have showed that C1orf124 protein binds monoubiquitin and polyubiquitin chains. Pull down assay showed that interaction is achieved through hydrophobic patch surrounding Ile44 on ubiquitin and Asp residue in-between second Zn-binding dyad of UBZ4 domain.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:353069
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-07 09:32:06