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master's thesis
Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:907353
Gogala, Marko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Gogala, M. (2009). Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:907353

Gogala, Marko. "Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:907353

Gogala, Marko. "Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:907353

Gogala, M. (2009). 'Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:907353

Gogala M. Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:907353

M. Gogala, "Strukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:907353

Metadata
TitleStrukturna i funkcijska analiza veznog mjesta kvaščevog proteina Sec13
Title (english)Structural and functional analysis of the yeast protein Sec13 with an qmphasis on its protein interaction interface
AuthorMarko Gogala
MentorThomas Schwartz (mentor)
Committee memberIvana Weygand-Đurašević (predsjednik povjerenstva)
Committee memberDunja Leljak-Levanić (član povjerenstva)
Committee memberDijana Škorić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Jezgra omeđena ovojnicom temeljna je osobina eukariota. Kako je kompleks jezgrine pore (NPC) jedini posrednik u transportu između jezgre i citoplazme, rasvjetljavanje njegove strukture važno je za razumijevanje eukariotskih stanica. Sec13 je komponenta NPC čija je uloga u održavanju i stabilizaciji kompleksa nejasna. Njegova dvojna uloga u vezanju proteina Nup145C u sklopu NPC te proteina Sec31 u sklopu vezikula tipa COPII otežava mutacijsku analizu. Cilj ovog projekta bio je riješiti strukturu Sec13 u kompleksu sa insercijskom regijom Nup145C, kako bi se dobila jasna slika veznog mjesta Sec13/Nup145C. Riješena struktura uspoređena je sa strukturama kompleksa Sec13/Sec31. Na temelju razlika među veznim mjestima osmišljen je mutirani Sec13 koji veže isključivo protein Sec31. Posljedica ove mutacije potvrđena je pokusima in vitro.
Abstract (english)
Possession of an envelope-coated nucleus is the defining trait of eukaryotes. Since the nuclear pore complex (NPC) is the only known mediator of nucleo-cytoplasmic traffic, elucidating its structure is important for understanding eukaryotic cells. Sec13 is a component of the NPC whose importance for the stability of the complex is unclear. Its dual role in binding proteins Nup145 in the NPC and Sec31 in COPII vesicles makes mutational analysis of Sec13 function difficult. The goal of this project was solving a structure of Sec13 in complex with the Nup145C insertion blade that would provide a clear view of the Sec13/Nup145C interface. The obtained structure was compared to the structures of the Sec13/Sec31 complex. Based on the differences between the interaction sites of the structures, a mutant Sec13 protein was designed, that binds specifically to Sec31, but has lost affinity for Nup145C. The effect of this mutation was confirmed by in vitro experiments.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:907353
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-08 11:17:42