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master's thesis
Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:523653
Lisica, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Lisica, A. (2009). Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:523653

Lisica, Ana. "Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:523653

Lisica, Ana. "Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:523653

Lisica, A. (2009). 'Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:523653

Lisica A. Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:523653

A. Lisica, "Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:523653

Metadata
TitlePrijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null
Title (english)Transport of APP protein in organelles of endocytic pathway in CHO NPC1-null cells
AuthorAna Lisica
MentorSilva Katušić Hećimović (mentor)
MentorDubravka Hranilović (sumentor)
Committee memberSilva Katušić Hećimović (predsjednik povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
Committee memberŽeljka Vidaković Cifrek (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Promijenjen metabolizam i povećana sinteza peptida amiloid β (Aβ) smatra se ključnim događajem u razvoju Alzheimerove bolesti (AB). Nedavna istraživanja su pokazala da razina kolesterola utječe na procesiranje prekursora proteina amiloid β (APP), te utječe na stvaranje i razinu peptida Aβ. Nakupljanje kolesterola i značajno povećanje razine peptida Aβ uočeno je i u Niemann Pickovoj bolesti tipa C (NPC), nasljednoj bolesti nakupljanja lipida, pa se ova bolest pokazala kao dobar model u proučavanju mehanizma djelovanja kolesterola na razvoj AB. Cilj rada je bio ispitati utječe li nakupljanje kolesterola u bolesti NPC na promijenjen smještaj proteina APP i presenilina 1 (PS1) u staničnim organelima endocitoznog puta. Korištenjem modelnih stanica CHO NPC1-/- i stanica divljeg tipa (CHOwt), pokazano je da stanice CHO NPC1-/- pokazuju promijenjen smještaj proteina APP u ranim i reciklirajućim endosomima. Stanice CHO NPC1-/- pokazuju promijenjeno bojanje biljega za rane i reciklirajuće endosome što ukazuje na njihovo nakupljanje na periferiji stanice. Međutim nije pokazan promijenjen smještaj proteina PS1 u organelima endocitoznog puta. Ovi rezultati ukazuju da bi kolesterol mogao djelovati na nastanak AB tako da regulira smještaj proteina APP unutar staničnih organela endocitoznog puta, te da takva preraspodjela proteina APP dovodi do povećanog stvaranja odsječka C99, te peptida Aβ u ovim organelima.
Abstract (english)
Altered metabolism and increased formation of amyloid-β peptide (Aβ) is considered to be the key event in development of Alzheimer's disease (AD). Recent reports have shown that cholesterol levels modulate processing of amyloid-β precursor protein (APP) and formation of Aβ peptide. Cholesterol accumulation and increased levels of Aβ peptide have also been observed in Niemann-Pick type C disease (NPC), hereditary sphingolipid storage disorder, which has become an important model to study the role of cholesterol in the pathogenesis of AD. The goal of this work was to investigate whether cholesterol accumulation in NPC disease changes distribution of APP protein and presenilin 1 (PS1) within endocytic pathway. Using immunoconfocal microscopy, we observed altered localization of APP protein in early and recycling endosomes in CHO NPC1-/- compared to CHOwt cells. In addition, we detected altered staining of markers for early (EEA1) and recycling (transferrin receptor, TFR) endosomes in CHO NPC1-/- cells, which suggests their accumulation on the cell periphery. However, altered localization of PS1 was not observed in endocytic organelles. These results suggest that cholesterol may influence the onset of AD by regulating trafficking of APP protein within endocytic pathway, and that this redistribution od APP protein leads to increased synthesis of C99, and Aβ peptide in these organelles.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:523653
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-13 11:06:13