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master's thesis
Analiza metagenoma ekstremnih okoliša
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:366208
Roller Milošević, Maša
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Roller Milošević, M. (2009). Analiza metagenoma ekstremnih okoliša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:366208

Roller Milošević, Maša. "Analiza metagenoma ekstremnih okoliša." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:366208

Roller Milošević, Maša. "Analiza metagenoma ekstremnih okoliša." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:366208

Roller Milošević, M. (2009). 'Analiza metagenoma ekstremnih okoliša', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:366208

Roller Milošević M. Analiza metagenoma ekstremnih okoliša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:366208

M. Roller Milošević, "Analiza metagenoma ekstremnih okoliša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:366208

Metadata
TitleAnaliza metagenoma ekstremnih okoliša
Title (english)Analysis of metagenomes in extreme environments
AuthorMaša Roller Milošević
MentorKristian Vlahoviček (mentor)
Committee memberMirjana Kalafatić (predsjednik povjerenstva)
Committee memberDijana Škorić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Samo je malen broj bakterija prisutnih u biosferi moguće uzgojiti u laboratorijskim uvjetima, i stoga sve veća količina znanja o raznolikosti genoma prokariota dolaze iz direktne analize DNA iz okoliša pomoću visokoprotočnih metoda skupnog naziva Metagenomika. Metagenomom se naziva skup svih gena nekog mikrobnog okoliša. Poznato je da prokariotski genomi pokazuju značajnu nejednakost u korištenju sinonimnih kodona (CUB) (prema eng. codon usage bias). CUB je izravno povezan s razinom ekspresije gena stoga što su kodoni koje koriste visokoeksprimirani geni komplementarni s najrasprostranjenijim tRNA u organizmu. Dakle, CUB gena se može povezati s razinom ekspresije gena kroz translacijsku optimizaciju. Analizom različitih metagenomskih uzoraka pokazali smo da je moguće primijetiti nejednako iskorištenje kodona unutar pojedinog mikrobnog okoliša, kao i da je takvo svojstvo moguće iskoristiti za predviđanje razine ekspresije gena na razini cjelokupnog mikrobnog ekosustava. Primjena ovog pristupa omogućuje istraživanje genske adaptacije mikrobnih organizama na okoliš.
Abstract (english)
Not all prokaryotes are amenable to cultivation in laboratory conditions, and increasing amounts of knowledge about microbial diversity is gained from direct sampling of DNA from environments. A rapidly developing field that studies whole microbial communities is metagenomics, the culture‐independent genomic study of organisms extracted directly from an ecological niche. Prokaryotic genomes show strong codon usage bias (CUB). CUB is directly correlated with expression levels of genes because codons used by genes expressed at high levels are encoded for by the most abundant tRNAs. Therefore, the CUB of a gene can be linked to its expression level through translational optimization. We show that metagenomes, much like genomes, also show CUB and that this phenomenon can be used to predict the expression level of genes at the level of the entire microbial community. We analyzed adaptation of organisms in metagenomes to their extreme environments through this approach.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:366208
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-16 11:50:38