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master's thesis
Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:643362
Dumić, Hrvoje
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Dumić, H. (2020). Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:643362

Dumić, Hrvoje. "Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:643362

Dumić, Hrvoje. "Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:643362

Dumić, H. (2020). 'Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS', Master's thesis, University of Zagreb, Faculty of Science, accessed 17 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:643362

Dumić H. Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 May 17] Available at: https://urn.nsk.hr/urn:nbn:hr:217:643362

H. Dumić, "Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:643362

Metadata
TitleIsolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS
Title (croatian)Izolacija i određivanje TG-estolida iz uzorka sirastog maza upotrebom TLC-a, HPLC-ESI/MS-a i GC-MS-a
AuthorHrvoje Dumić
MentorJozef Cvačka https://orcid.org/0000-0002-3590-9009 (mentor)
Committee memberPredrag Novak (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberIvana Biljan (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-01-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
A complete isolation of vernix caseosa triacylglycerol estolides with combination of thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), and their partial structural elucidation with high-performance liquid chromatography – mass spectrometry (HPLC-MS) and gas chromatography – mass spectrometry (GC-MS) hyphenated systems was performed. New semi-preparative TLC method for isolation and semi-preparative HPLC method for additional purification of vernix caseosa triacylglycerol estolides were developed. Structural analysis of intact triacylglycerol estolides with LC-MS system revealed 126 structural isomers and after transesterification of triacylglycerol estolides, analysis of fatty acid methyl esters with GC-MS 71 different fatty acids that build vernix caseosa triacylglycerol estolides were observed.
Abstract (croatian)
Upotrebom tankoslojne kromatografije (TLC) i tekućinske kromatografije visoke djelotvornosti (HPLC) provedena je potpuna izolacija triacilglicerol estolida iz uzorka sirastog maza (vernix caseosa) te je djelomično određena njihova struktura upotrebom vezanih sustava tekućinska kromatografija visoke djelotvornosti – masena spektrometrija (HPLC-MS) i plinska kromatografija – masena spektrometrija (GC-MS). Razvijena je nova metoda semi-preparativne tankoslojne kromatografije za izolaciju i nova metoda semi-preparativne tekućinske kromatografije visoke djelotvornosti za dodatno pročišćavanje triacilglicerol estolida iz uzorka sirastog maza. Strukturna analiza triacilglicerol estolida, u njihovom intaktnom obliku, upotrebom vezanog sustava HPLC-MS otkrila je postojanje 126 strukturnih izomera. Također je, nakon transesterifikacije uzorka, strukturnom analizom metilnih estera masnih kiselina upotrebom vezanog sustava GC-MS otkriveno 71 različitih masnih kiselina koje grade triacilglicerol estolide u sirastom mazu.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:643362
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-02-06 15:28:14