prikaz prve stranice dokumenta Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores
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Scientific paper - Original scientific paper
Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores
Nature Communications, 7 (2016); 10298. https://doi.org/10.1038/ncomms10298
Kajtez, Janko; Solomatina, Anastasia; Novak, Maja; Polak, Bruno; Vukušić, Bruno; Rüdiger, Jonas; Cojoc, Gheorghe; Milas, Ana; Šumanovac Šestak, Ivana; Risteski, Patrik; Tavano, Federica; Klemm, Anna H.; Roscioli, Emanuele; Welburn, Julie; Cimini, Daniela; Glunčić, Matko; Pavin, Nenad; Tolić, Iva Marija More authors...

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Kajtez, J., Solomatina, A., Novak, M., Polak, B., Vukušić, B., Rüdiger, J. ... Tolić, I. M. (2016). Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores. Nature Communications, 7.. doi: 10.1038/ncomms10298

Kajtez, Janko, et al. "Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores." Nature Communications, vol. 7, 2016. https://doi.org/10.1038/ncomms10298

Kajtez, Janko, Anastasia Solomatina, Maja Novak, Bruno Polak, Bruno Vukušić, Jonas Rüdiger, Gheorghe Cojoc, et al. "Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores." Nature Communications 7 (2016). https://doi.org/10.1038/ncomms10298

Kajtez, J., et al. (2016) 'Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores', Nature Communications, 7. doi: 10.1038/ncomms10298

Kajtez J, Solomatina A, Novak M, Polak B, Vukušić B, Rüdiger J, and sur.. Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores. Nature Communications [Internet]. 2016 [cited 2024 December 29];7. doi: 10.1038/ncomms10298

J. Kajtez, et al., "Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores", Nature Communications, vol. 7, 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:208275. [Accessed: 29 December 2024]

Metadata
Title (english)Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores
AuthorJanko Kajtez
AuthorAnastasia Solomatina
AuthorMaja Novak
MBZ: 344456
AuthorBruno Polak
AuthorBruno Vukušić
AuthorJonas Rüdiger
AuthorGheorghe Cojoc
AuthorAna Milas
AuthorIvana Šumanovac Šestak
AuthorPatrik Risteski
AuthorFederica Tavano
AuthorAnna H. Klemm
AuthorEmanuele Roscioli
AuthorJulie Welburn
AuthorDaniela Cimini
AuthorMatko Glunčić
AuthorNenad Pavin
AuthorIva Marija Tolić
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract (english)
During metaphase, forces on kinetochores are exerted by k-fibres, bundles of microtubules that end at the kinetochore. Interestingly, non-kinetochore microtubules have been observed between sister kinetochores, but their function is unknown. Here we show by laser-cutting of a k-fibre in HeLa and PtK1 cells that a bundle of non-kinetochore microtubules, which we term ‘bridging fibre’, bridges sister k-fibres and balances the interkinetochore tension. We found PRC1 and EB3 in the bridging fibre, suggesting that it consists of antiparallel dynamic microtubules. By using a theoretical model that includes a bridging fibre, we show that the forces at the pole and at the kinetochore depend on the bridging fibre thickness. Moreover, our theory and experiments show larger relaxation of the interkinetochore distance for cuts closer to kinetochores. We conclude that the bridging fibre, by linking sister k-fibres, withstands the tension between sister kinetochores and enables the spindle to obtain a curved shape.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleNature Communications
Numberingvol. 7, 10298
e-ISSN2041-1723
DOIhttps://doi.org/10.1038/ncomms10298
URN:NBNurn:nbn:hr:217:208275
Publication2016
Document URLhttps://www.nature.com/articles/ncomms10298
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
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Created on2020-02-09 14:31:04