prikaz prve stranice dokumenta Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
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Scientific paper - Original scientific paper
Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
Journal of Cell Biology, 212 (2016), 7; 767-776. https://doi.org/10.1083/jcb.201506011
Cojoc, Gheorghe; Roscioli, Emanuele; Zhang, Lijuan; García-Ulloa, Alfonso; Shah, Jagesh V.; Berns, Michael W.; Pavin, Nenad; Cimini, Daniela; Tolić, Iva Marija; Gregan, Juraj

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Cojoc, G., Roscioli, E., Zhang, L., García Ulloa, A., Shah, J. V., Berns, M. W. ... Gregan, J. (2016). Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. Journal of Cell Biology, 212. (7), 767-776. doi: 10.1083/jcb.201506011

Cojoc, Gheorghe, et al. "Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore." Journal of Cell Biology, vol. 212, no. 7, 2016, pp. 767-776. https://doi.org/10.1083/jcb.201506011

Cojoc, Gheorghe, Emanuele Roscioli, Lijuan Zhang, Alfonso García Ulloa, Jagesh V. Shah, Michael W. Berns, Nenad Pavin, Daniela Cimini, Iva Marija Tolić and Juraj Gregan. "Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore." Journal of Cell Biology 212, no. 7 (2016): 767-776. https://doi.org/10.1083/jcb.201506011

Cojoc, G., et al. (2016) 'Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore', Journal of Cell Biology, 212(7), pp. 767-776. doi: 10.1083/jcb.201506011

Cojoc G, Roscioli E, Zhang L, García Ulloa A, Shah JV, Berns MW, and sur.. Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. Journal of Cell Biology [Internet]. 2016 [cited 2024 November 25];212(7):767-776. doi: 10.1083/jcb.201506011

G. Cojoc, et al., "Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore", Journal of Cell Biology, vol. 212, no. 7, pp. 767-776, 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:574387. [Accessed: 25 November 2024]

Metadata
Title (english)Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore
AuthorGheorghe Cojoc
AuthorEmanuele Roscioli
AuthorLijuan Zhang
AuthorAlfonso García-Ulloa
AuthorJagesh V. Shah
AuthorMichael W. Berns
AuthorNenad Pavin
MBZ: 204800
AuthorDaniela Cimini
AuthorIva Marija Tolić
MBZ: 260543
AuthorJuraj Gregan
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract (english)
Accurate chromosome segregation depends on proper kinetochore– microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore, which is laterally stretched by opposing pulling forces exerted by microtubules, and inferred the mechanical response of the kinetochore from its length change. In both mammalian PtK1 cells and in the fission yeast Schizosaccharomyces pombe, kinetochores shortened after microtubule severing. Interestingly, the inner kinetochore–centromere relaxed faster than the outer kinetochore. Whereas in fission yeast all kinetochores relaxed to a similar length, in PtK1 cells the more stretched kinetochores remained more stretched. Simple models suggest that these differences arise because the mechanical structure of the mammalian kinetochore is more complex. Our study establishes merotelic kinetochores as an experimental model for studying the mechanical response of the kinetochore in live cells and reveals a viscoelastic behavior of the kinetochore that is conserved in yeast and mammalian cells.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Cell Biology
Numberingvol. 212, no. 7, pp. 767-776
p-ISSN0021-9525
e-ISSN1540-8140
DOIhttps://doi.org/10.1083/jcb.201506011
URN:NBNurn:nbn:hr:217:574387
Publication2016
Document URLhttps://rupress.org/jcb/article/212/7/767/38423/Laser-microsurgery-reveals-conserved-viscoelastic
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) icon
Public note© 2016 Cojoc et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). Submitted: June 2, 2015. Accepted: February 25, 2016. Published March 21, 2016. Šifre projekata: RGY0069/2010, MCB- 0842551, MCB-1517506, P21437, P23609, EC (PCIG11-GA-2012-322300), APVV (APVV- 0334-12), VEGA (1/0196/14)
Created on2020-02-23 13:39:31