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master's thesis
Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:301132
Dragun, Josipa
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Dragun, J. (2016). Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:301132

Dragun, Josipa. "Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:301132

Dragun, Josipa. "Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:301132

Dragun, J. (2016). 'Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:301132

Dragun J. Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:301132

J. Dragun, "Učinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:301132

Metadata
TitleUčinkovitost galne kiseline na angiogenezu, oksidativni stres i rast tumora
Title (english)The efficiency of gallic acid on angiogenesis, oxidative stress and tumor growth
AuthorJosipa Dragun
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Formiranje tumora je proces koji uključuje akumuliranje genetskih i epigenetskih promjena i vodi do progresivne transformacije normalnih, zdravih stanica u maligne. Tumorske stanice posjeduju određene sposobnosti nepoznate normalnim stanicama: otpornost na inhibiciju rasta, proliferiraju bez ovisnosti o faktorima rasta, neograničeno se repliciraju, izbjegavaju apoptozu, invadiraju i metastaziraju te podupiru angiogenezu. Razvoj tumorske bolesti usko je povezan i s utjecajem reaktivnih kisikovih spojeva (ROS) na brojne stanične funkcije. U radu smo istražili utjecaj galne kiseline (GA) na oksidacijski stres, rast tumora i angiogenezu u miševa nositelja Ehrlichovog ascitesnog tumora (EAT). EAT stanice (2,5 x 106) injicirane su intraperitonealno (ip) u Swiss albino miševe. Nakon inokulacije tumora, miševi su injicirani ip s GA u koncentraciji od 40 i 80 mg/kg tjelesne težine u fazi eksponencijalnog rasta od 5 dana nakon injiciranja stanica tumora peti, sedmi i deveti dan i jedanaesti dan. Ukupni broj stanica ascitesa u peritonealnoj šupljini, diferencijalnu analizu stanica prisutnih u peritonealnoj šupljini, volumen ascitesa, funkcionalnu sposobnost makrofaga te druge protutumorske karakteriste GA analizirali smo 13-ti dan. Rezultatima je pokazano da je GA inhibirala rast EAT stanica i nastanak ascitesa u peritonealnoj šupljini miševa nositelja EAT. Antiangiogeni potencijal GA in vivo pokazan je kroz rezultate smanjene peritonealne angiogeneze i mikrožilne gustoće. Nadalje, GA je smanjila razinu NO u stanicama EAT, ali je povećala razinu NO u peritonealnim makrofagima. Razina arginaze 1 bila je snižena što ukazuje da je spriječena polarizacija u M2 makrofage. Na osnovu dobivenih rezultata možemo zaključiti da GA može aktivirati makrofage i povećati njihovo citotoksično djelovanje kroz povećanu proizvodnju NO, te spriječiti rast tumora i angiogenezu.
Abstract (english)
Tumor formation is a process that involves accumulation of genetic and epigenetic changes leading to the progressive transformation of normal, healthy cells into malignant. Tumor cells possess certain skills unknown to normal cells: survival, proliferation without dependence on growth factors, unlimited replication, avoidance of apoptosis, invasivnes, metastasis and angiogenesis. The development of tumorogenesis is closely associated with the influence of reactive oxygen species (ROS) at the variety of cellular functions. In the present study we investigated the effect of of gallic acid on oxidative stress, tumor growth and angiogenesis in EAT- bearing mice. EAT cells (2.5 x 106) were implanted intraperitoneally (ip) in Swiss albino mice. After tumor inoculation, mice were injected ip with GA at dose of 40 and 80 mg/kg bw in exponential growth phase from the 5 days after tumor cell injection (on day 5, 7, 9,11). On day 13, ascites volume, the total number of cells, differential count of the cells present in the peritoneal cavity, functional activity of macrophages, anti-angiogenic and antioxidant parameters were determined. Results show that GA inhibited the growth of EAT cells and the formatin of ascites in the peritoneal cavity of EAT-bearing mice. Further, results on decrease in the peritoneal angiogenesis and microvessel density show the anti-angiogenic potential of CA in vivo. Gallic acid also decreased NO level in tumor cells whereas NO level was increased in peritoneal macrophages. Decreased level of Arginase 1 (ARG 1) indicate that polarization is prevented in M2 macrophages. Based on the results we can conclude that the GA can activated macrophages and increase their cytotoxic activity through increased production of NO and prevent tumor growth and angiogenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:301132
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-27 12:41:11