prikaz prve stranice dokumenta Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela
  Download
PDF 1.97 MB
master's thesis
Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2019. urn:nbn:hr:109:839493
Barišić, Iva
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Barišić, I. (2019). Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:839493

Barišić, Iva. "Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2019. https://urn.nsk.hr/urn:nbn:hr:109:839493

Barišić, Iva. "Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2019. https://urn.nsk.hr/urn:nbn:hr:109:839493

Barišić, I. (2019). 'Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:109:839493

Barišić I. Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:109:839493

I. Barišić, "Lipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:109:839493

Metadata
TitleLipaze iz Pseudomonas fluorescens i Burkholderia cepacia kao biokatalizatori u sintezi biodizela
Title (english)Lipase from Pseudomonas fluorescens and Burkholderia cepacia as Biocatalysts in Biodiesel Synthesis
AuthorIva Barišić
MentorSandra Budžaki (mentor)
MentorMarta Ostojčić (pomoć pri izradi, neposredni voditelj)
Committee memberIvica Strelec (predsjednik povjerenstva)
Committee memberSandra Budžaki (član povjerenstva)
Committee memberNatalija Velić (član povjerenstva)
Committee memberIvana Flanjak (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Thermodynamics and Reaction Engineering)
Osijek
Defense date and country2019-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
U današnjoj industrijskoj proizvodnji biodizela kao katalizator koristi se natrijev hidroksid, no ovaj način proizvodnje ima brojne nedostatke. Stoga se sve više istražuju alternativni oblici proizvodnje biodizela, među kojima se ističe upotreba biokatalizatora ‐ lipaza. U usporedbi s kemijski kataliziranom, reakcija katalizirana lipazama je jednostavnija i ekološki prihvatljivija. U ovom radu provedena je biotehnološka karakterizacija Amano lipaza iz bakterija Burkholderia cepacia i Pseudomonas fluorescens primjenom dva različita testa, spektrofotometrijskog uz p‐nitrofenil palmitat kao supstrat i titrimetrijskog testa uz maslinovo ulje kao susptrat te je procijenjeno kako se primjena eksperimentalno određenog optimalnog pH i optimalne temperature odražava na učinkovitost biokatalitičke sinteze biodizela u kotlastom bioreaktoru uz svježe suncokretovo ulje i metanol kao supstrate u reakcijama transesterifikacije navedenim lipazama. Potom je udio metilnih estera masnih kiselina u sirovom biodizelu određen na plinskom kromatografu te je na temelju dobivenih rezultata procijenjeno da lipaza iz Burkholderia cepacia pokazuje veću efikasnost u proizvodnji biodizela.
Abstract (english)
In today's industrial production of biodiesel, sodium hydroxide is being used as a catalyst, but this production method has numerous disadvantages. Therefore, alternative forms of biodiesel production are being explored, among which the use of lipase as biocatalysts is emphasized. Compared to chemically catalyzed, the lipase catalyzed reaction is simpler and more environmentally friendly. In this master thesis, biotechnological characterization of Amano lipases from Burkholderia cepacia and Pseudomonas fluorescens bacterias was conducted, by using two different tests, spectrophotometric with p‐nitrophenyl palmitate as a substrate and a titrimetry test with olive oil as a substrate, and it was estimated that using experimentally specified optimal pH and optimal temperature reflects on efficiency of biocatalitic sinthesys of biodiesel in batch bioreactor with fresh sunflower oil and methanol as supstrates in transesterification reaction with listed lipases. Afterwards, the proportion of fatty acid methyl esters in crude biodiesel was determined on a gas chromatograph and, based on the obtained results, it was estimated that lipase from Burkholderia cepacia showed a higher efficiency in biodiesel production.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:839493
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2019-09-19 14:07:00