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undergraduate thesis
Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2020. urn:nbn:hr:109:770973
Pilić, Daria
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Pilić, D. (2020). Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:770973

Pilić, Daria. "Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2020. https://urn.nsk.hr/urn:nbn:hr:109:770973

Pilić, Daria. "Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2020. https://urn.nsk.hr/urn:nbn:hr:109:770973

Pilić, D. (2020). 'Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 08 November 2024, https://urn.nsk.hr/urn:nbn:hr:109:770973

Pilić D. Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2020 [cited 2024 November 08] Available at: https://urn.nsk.hr/urn:nbn:hr:109:770973

D. Pilić, "Detekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:109:770973

Metadata
TitleDetekcija aktivnosti lipaza imobiliziranih na polivinilidendifluoridnoj membrani
Title (english)Detection of Lipase Activity Immobilized on a PVDF Membrane
AuthorDaria Pilić
MentorIvica Strelec (mentor)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Biochemistry and Toxicology)
Osijek
Defense date and country2020-09-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Cilj ovog završnog rada bio je odrediti mogućnost detekcije i kvantifikacije aktivnosti lipaza imobiliziranih adsorpcijom na PVDF membranu primjenom „dot-blot“ tehnike. Za detekciju aktivnosti imobiliziranih lipaza podrijetlom iz bakterija Burkholderia cepacia i Pseudomonas fluorescens korišteni su α-naftilacetat kao supstrat, te dvije različite azo boje Fast Red TR i Fast Blue B sol kao kompleksirajući reagensi. Kvantifikacija aktivnosti imobiliziranih lipaza provedena je primjenom modula Protein Array Analyzer programa ImageJ. Rezultati su pokazali da je moguće provesti kvantifikaciju aktivnosti lipaza imobiliziranih na PVDF membranu primjenom Fast Red TR sol i Fast Blue B soli uz α-naftil-acetat kao supstrat. Međutim za potpunu kvantifikaciju nanesene lipazne aktivnosti potrebno je na PVDF membranu nanositi od 1-100 mU za detekciju aktivnosti B.cepaica lipaze te aktivnost od 1-30 mU za detekciju aktivnosti P. fluorescens lipaze uz primjenu Fast Red TR soli, dok se u slučaju azo boje Fast Blue B ovaj raspon za obje lipaze kreće od 1- 25 mU.
Abstract (english)
The aim of this undergraduate thesis was to determine the possibility of detection and quantification of activity of lipases immobilized by adsorption onto PVDF membrane by „dot-blot“ technique. Activity detection of Burkholderia cepacia and Pseudomonas fluorescens lipases immobilized on PVDF membrane was performed with α-naphthylacetate as lipase substrate and two different azo dyes, Fast Red TR and Fast Blue B salt, as complexing reagents, while Protein Array Analyzer module of ImageJ software for immobilized lipase activity quantification. Obtained results revealed the possibility for detection of PVDF membrane immobilized lipase activity using both azo dyes, Fast Red TR and Fast Blue B salt. However, in order to ensure complete quantification of immobilized lipase activities, it will be necessary to apply from 1 to 100 mU of B. cepacia lipase per dot onto PVDF membrane and from 1 to 30 mU of P. fluorescens lipase when using Fast Red TR salt as copulating reagent, while in case of Fast Blue B salt from 1 to 25 mU of both lipases.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:770973
Study programmeTitle: Food Technology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2020-09-07 11:47:53