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master's thesis
Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2021. urn:nbn:hr:109:887428
Kenjerić, Lidija
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Biochemistry and Toxicology

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Kenjerić, L. (2021). Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:887428

Kenjerić, Lidija. "Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2021. https://urn.nsk.hr/urn:nbn:hr:109:887428

Kenjerić, Lidija. "Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2021. https://urn.nsk.hr/urn:nbn:hr:109:887428

Kenjerić, L. (2021). 'Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 17 February 2025, https://urn.nsk.hr/urn:nbn:hr:109:887428

Kenjerić L. Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2021 [cited 2025 February 17] Available at: https://urn.nsk.hr/urn:nbn:hr:109:887428

L. Kenjerić, "Development and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:109:887428

Metadata
TitleDevelopment and Validation of an HPLC-MS/MS Multi-Class Method with Special Emphasis on Pharmacologically Active Agents in Milk and Chicken Feed
Title (croatian)Razvoj i validacija HPLC-MS/MS metode za višeklasnu analizu s posebnim naglaskom na farmakološki aktivne tvari u mlijeku i hrani za perad
AuthorLidija Kenjerić
MentorTomislav Klapec (mentor)
MentorMichael Sulyok https://orcid.org/0000-0002-3302-0732 (pomoć pri izradi, neposredni voditelj)
Committee memberIvana Flanjak (predsjednik povjerenstva)
Committee memberTomislav Klapec (član povjerenstva)
Committee memberIvana Tomac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Biochemistry and Toxicology)
Osijek
Defense date and country2021-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
U ovom je radu razvijana višeklasna metoda visokotlačne tekućinske kromatografije tandemske masene spektrometrije (HPLC-MS/MS) za analizu farmakološki aktivnih tvari, odnosno različitih klasa ostataka veterinarskih lijekova u mlijeku i hrani za perad, koja je naposljetku i validirana. Metoda korištena u ovoj studiji bila je modifikacija pristupa koji su opisali Malachová i sur. (2014). Ova je metoda prenesena i utvrđeni su LC-MS parametri antibiotskih i antimikrobnih spojeva. Omjer m/z za sve optimizirane analite određen je u pozitivnom i u negativnom ionizacijskom načinu. Optimizirani parametri implementirani su u „novu“multi metodu. Potom je testirana stabilnost standarda, dok je za potrebe optimizacije ekstrakcijske procedure ispitana uporaba različitih otapala. Prije analize, uzorci mlijeka i hrane za perad podvrgnuti su ekstrakciji u kiselim i neutralnim ekstrakcijskim uvjetima. Metoda je validirana ispitivanjem sljedećih parametara izvedbe: linearnost, LOD, LOQ, preciznost, točnost i unutarlaboratorijska ponovljivost. Rezultati validacije metode pokazuju da usprkos velikog opsega ispitivanih analita u smislu polarnosti, stabilnosti i topljivosti, velik dio analita (80-90%) udovoljava SANTE kriterijima prema kojima je validacija provedena.
Abstract (english)
In this work, a multi-class high-pressure liquid chromatography - tandem mass spectrometry (HPLC-MS / MS) method was developed and validated for the analysis of pharmacologically active substances, i.e., different classes of veterinary drug residues in milk and poultry feed. The method used in this study was a modification of the approach described by Malachová et al. (2014). The m/z ratio for all optimized analytes were detected both in the ESI positive and negative mode and the more sensitive mode was selected. The m/z ratio for all optimized analytes was determined in both positive and negative ionization modes. Optimized parameters have been implemented in a "new" multimethod. The stability of standards was tested, and different solvents were tested for the purpose of optimizing the extraction procedure. Prior to analysis, milk and poultry feed samples were subjected to extraction under acidic and neutral extraction conditions. The method was validated through examination of the following performance parameters: linearity, LOD, LOQ, precision, accuracy and within laboratory repeatability. The results of the method validation show that despite the large range of tested analytes in terms of polarity, stability and solubility, a large part of the analytes (80-90%) meet the SANTE validation criteria.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:887428
Study programmeTitle: Food Science and Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani (magistar/magistra inženjer/inženjerka nutricionizma i znanosti o hrani)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2021-09-02 09:54:22