Ultrasound-assisted extraction of pigments (UAE) from the green macroalga Codium bursa was performed with the aim of optimizing the extraction parameters. With methanol:dichloromethane as extraction solvent, the following conditions were investigated: temperature (30, 50 and 70 °C), time (15, 30 and 45 min) and solvent:solid ratio (10, 20 and 30 mL/g). Based on response surface methodology, 17 experiments were performed, in which the influence of process parameters on concentrations of tested pigments was monitored. Fucoxanthin concentrations in obtained extracts were determined by high-performance liquid chromatography (HPLC) and ranged from 0.07 to 0.43 mg/g of dry extract and were higher than in the extracts obtained by the conventional method (0.18 ± 0.09 mg/g dry extract). Linear and quadratic terms of time had statistically highly significant influence on fucoxanthin extraction, and its highest concentration was obtained at the following conditions: 30.29 °C, 15.06 min, 15.17 mL/g. Concentrations of other pigments were determined spectrofotometrically and ranged from 0.0336 to 0.3762 mg/g for chlorophyll b, 0.0067 to 0.0685 mg/g for chlorophyll a, and 0.0038 to 0. 0467 mg/g dry extract for total carotenoids. Linear term of temperature and solvent:solid ratio had statistically highly significant influence on extractability of chlorophyll b and total carotenoids; for chlorophyll a those and other parameters had a significant influence. Their highest concentrations were obtained at the conditions: 30°C, 30 min and 10 mL/g.