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undergraduate thesis
Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. urn:nbn:hr:109:842043
Šarić, Dario
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Chemistry and Ecology

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Šarić, D. (2022). Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:842043

Šarić, Dario. "Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. https://urn.nsk.hr/urn:nbn:hr:109:842043

Šarić, Dario. "Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. https://urn.nsk.hr/urn:nbn:hr:109:842043

Šarić, D. (2022). 'Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 15 May 2024, https://urn.nsk.hr/urn:nbn:hr:109:842043

Šarić D. Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2022 [cited 2024 May 15] Available at: https://urn.nsk.hr/urn:nbn:hr:109:842043

D. Šarić, "Izolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:109:842043

Metadata
TitleIzolacija bioaktivnih komponenti iz ekstrakata alge Cystoseira compressa primjenom makroporoznih smola
Title (english)Isolation of Bioactive Components from Cystoseira compressa Alga Extracts Using Macroporous Resins
AuthorDario Šarić
MentorMaja Molnar (mentor)
MentorMartina Jakovljević Kovač (pomoć pri izradi, neposredni voditelj)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Chemistry and Ecology)
Osijek
Defense date and country2022-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Cystoseira compressa je jedna od najrasprostanjenijih algi na području Jadranskog mora. Riječ je o smeđoj algi koja sintetizira širok spektar sekundarnih metabolita koji su biološki aktivni. Takvi spojevi imaju pozitivan utjecaj na zdravlje ljudi te zbog toga navedena alga ima velik farmakološki potencijal. U budućnosti, značajna može biti i u pogledu funkcionalne hrane. Za ekstrakciju bioaktivnih komponenti, sve je češća primjena tzv. niskotemperaturnih eutektičkih otapala koja su i dalje u fazi istraživanja. Problem kod eutektičkih otapala pojavljuje se prilikom analize ekstrahiranih spojeva prvenstveno zbog viskoznosti samih otapala. U ovom radu, kao potencijalno rješenje izolacije i kvantificiranja ekstrahiranih spojeva u niskotemperaturnim eutektičkim otapalima, ispitana je metoda izolacije s makroporoznim smolama koje imaju ulogu adsorbensa. Izolacijom su biološki aktivni spojevi desorbirani iz niskotemperaturnog eutektičkog otapala (ekstrakta alge) u otapalo pogodno za daljnje analize (etanol). Izolirani spojevi ispitivani su primjenom spektrofotometrijskih i kromatografskih tehnika kako bi se ispitala prisutnost florotanina, ukupnih fenola, pigmenata i lakohlapljivih komponenata u etanolnom pripravku. Na temelju dobivenih rezultata, utvrđeno je da se makroporoznim smolama mogu izolirati samo pojedine komponente iz niskotemperaturnih eutektičkih otapala, točnije florotanini i ukupni fenoli, a kao najpogodnije eutektičko otapalo iz kojeg su se mogle izolirati iste, pokazalo se otapalo sastava fruktoza:mliječna kiselina u molarnom omjeru 1:5.
Abstract (english)
Cystoseira compressa is one of the most widespread algae in the Adriatic Sea. It is a brown alga that synthesizes a broad spectrum of biologically active secondary metabolites. Such compounds positively affect human health, which is why the mentioned alga has an excellent pharmacological potential. In the future, it may also be significant in terms of functional food. For the extraction of bioactive compounds, the usage of the so-called deep eutectic solvents, which are still being examined, is getting more pronounced. The main dissadvantage of deep eutectic solvents appears during the analysis of extracted compounds and is due to the viscosity of the solvents. In this paper, the isolation method with macroporous resins that act as adsorbents was examined as a potential solution for isolation and quantification of extracted compounds in deep eutectic solvents. Biologically active compounds were desorbed from deep eutectic solvent (alga extract) to a solvent suitable for further analysis (ethanol). The isolated compounds were examined using spectrophotometric and chromatographic techniques to examine the presence of phlorotannins, total phenols, pigments, and volatile components in the ethanol preparation. The results showed that macroporous resins could isolate only specific compounds from deep eutectic solvents, phlorotannins and total phenols namely. The most suitable eutectic solvent for their isolation was found to be a solvent composed of fructose:lactic acid in a molar ratio of 1:5.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:842043
Study programmeTitle: Food Technology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2022-09-27 07:45:45