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master's thesis
Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. urn:nbn:hr:109:779561
Grgić, Kristina
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Process Design and Construction Materials

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Grgić, K. (2023). Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:779561

Grgić, Kristina. "Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. https://urn.nsk.hr/urn:nbn:hr:109:779561

Grgić, Kristina. "Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2023. https://urn.nsk.hr/urn:nbn:hr:109:779561

Grgić, K. (2023). 'Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:109:779561

Grgić K. Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:109:779561

K. Grgić, "Primjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:109:779561

Metadata
TitlePrimjena ekstrakcije na čvrstoj fazi u izolaciji pigmenata iz smeđe makroalge Cystoseira corniculata
Title (english)Application of Solid Phase Extraction in the Isolation of Pigments from Brown Macroalga Cystoseira corniculata
AuthorKristina Grgić
MentorStela Jokić (mentor)
MentorKrunoslav Aladić (komentor)
MentorAna-Marija Cikoš (pomoć pri izradi, neposredni voditelj)
Committee memberDarko Velić (predsjednik povjerenstva)
Committee memberStela Jokić (član povjerenstva)
Committee memberKrunoslav Aladić (član povjerenstva)
Committee memberIvana Flanjak (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Process Design and Construction Materials)
Osijek
Defense date and country2023-06-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Provedena je ekstrakcija na čvrstoj fazi (SPE) na liofiliziranom uzorku smeđe makroalge Cystoseira corniculata. Ekstrakcijom su dobivene četiri frakcije; frakcija 1 (F1) i frakcija 2 (F2) služile su za pročišćavanje analita od interesa – pigmenata, koji su prikupljeni u frakciji 3 (F3) i 4 (F4). Dobivene frakcije F3 i F4 podvrgnute su tekućinskoj kromatografiji visoke djelotvornosti (HPLC) kako bi se izvršila identifikacija i kvantifikacija pigmenata te su spektrofotometrijski određene koncentracije ukupnih pigmenata. Radi usporedbe rezultata, navedeno je provedeno i na dodatne dvije vrste iz istog roda; Ericaria crinita i Gongolaria barbata. Kod sve tri vrste makroalgi zabilježen je fukoksantin kao glavni pigment u frakciji F3, a klorofil a kao glavni u frakciji F4. Od ukupnih karotenoida, najveću koncentraciju imala je C. corniculata, a iznosila je 0,67 mg/g suhog ekstrakta. G. barbata je sadržavala najviše klorofila a, u koncentraciji od 0,80 mg/g suhog ekstrakta, kao i klorofila b: 0,75 mg/g suhog ekstrakta. Za vrstu C. corniculata provedena je i 2,2-difenil-1-pikrilhidrazil (DPPH) metoda te je dokazano antioksidacijsko djelovanje s postotkom inhibicije DPPH radikala od 80,18 % u frakciji F3.
Abstract (english)
Solid phase extraction (SPE) was performed on a lyophilized sample of the brown macroalga Cystoseira corniculata. Four fractions were obtained by extraction; fraction 1 (F1) and fraction 2 (F2) were used to purify the analytes of interest - pigments, which were collected in fractions 3 (F3) and 4 (F4). The obtained fractions F3 and F4 were subjected to high performance liquid chromatography (HPLC) in order to identify and quantify the pigments, and the concentrations of total pigments were determined spectrophotometrically. In order to compare the results, the above was also carried out on two additional species from the same genus; Ericaria crinita and Gongolaria barbata. In all three types of macroalgae, fucoxanthin was recorded as the main pigment in the fraction F3, and chlorophyll a as the main pigment in the fraction F4. Of the total carotenoids, C. corniculata had the highest concentration, amounting to 0.67 mg/g of dry extract. G. barbata contained the most chlorophyll a, in a concentration of 0.80 mg/g dry extract, as well as chlorophyll b: 0.75 mg/g dry extract. For the species C. corniculata, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was also carried out, and the antioxidant activity was proven with a DPPH radical inhibition percentage of 80.18% in the fraction F3.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:779561
ProjectNumber: KK.01.1.1.01.0002
Title: Bioprospecting Jadranskog mora
Leader: Rozelindra Čož Rakovac
Jurisdiction: eu
Funder: Europska unija
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) icon
Created on2023-06-13 14:37:53