Abstract | U radu je istražen utjecaj probiotičkih fermentiranih napitaka od kravljeg, kozjeg i sojinog mlijeka
sa dodatkom bagremovog i kestenovog meda prema patogenoj bakteriji Yersinia enterocolitica.
Za inokulaciju mlijeka korištena je monokultura Bifidobacterium longum.
Za pripremu probiotičkih napitaka korišteno je UHT sterilizirano kravlje mlijeko s 2,8% mliječne
masti, UHT sterilizirano kozje mlijeko s 3,2% mliječne masti, te sojino mlijeko s 2,2% masti, a
med je dodavan u udjelima od 3%, 5% i 10%. Fermentacija se provodila na 37 ºC, a sva mjerenja
su se provodila 0 (nulti), te nakon 5, 10, 15, 20, 25 sati. Praćena je promjena aktivne kiselosti,
titracijske kiselosti, te promjena broja probiotičkih bakterija. Utvrđen je povoljan utjecaj dodatka
meda na brzinu fermentacije kravljeg, kozjeg i sojinog mlijeka, pri čemu je utjecaj bagremovog
meda bio izražajniji od kestenovog meda. Probiotički napitak od kozjeg mlijeka s dodatkom
bagremovog meda je pokazao jači inhibitorni učinak u usporedbi s probiotičkim napitkom od
kravljeg i sojinog mlijeka s dodatkom bagremovog meda, a pri tome je inhibicijski učinak jače bio
izražen kod udjela meda od 10%. |
Abstract (english) | The influence of probiotic fermented milk beverages of cow, goat and soy milk with acacia and chestnut honey addition against pathogenic Yersinia enterocolitica was examined in this work. For the milk inoculation monoculture Bifidobacterium longum was used. Probiotic drink was
produced from UHT sterilized cow milk with 2.8% milk fat, UHT sterilized goat milk with 3.2% milk fat and UHT sterilized soy milk with 2.2% fat. Honey was added in content of 3, 5 and 10 percentages. The fermentation was conducted on 37 °C and all the measurements were after 5, 10,
15, 20, and 25 hours. pH-value, titration acidity and changes of number probiotic bacterium were measured, respectively. Addition of honey had high influence on course and of fermentation cow, goat and soy milk. Acacia honey had stronger influence in comparison with chestnut honey.
The probiotic potion of goats milk with added acacia honey had a greater inhibitory effect than probiotic potion of cows or soya milk with added acacia honey, where the inhibitory effect was greater with 10% proportion of honey. |