prikaz prve stranice dokumenta Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava
Access restricted to students and staff of home institution
master's thesis
Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2015. urn:nbn:hr:178:723891
Butković, Ivan
University of Zagreb
Faculty of Veterinary Medicine
Department of Hygiene and Technology of Foodstuffs

Institutional repository: Repository of Faculty of Veterinary Medicine

Cite this document

Butković, I. (2015). Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:723891

Butković, Ivan. "Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:178:723891

Butković, Ivan. "Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:178:723891

Butković, I. (2015). 'Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 03 April 2025, https://urn.nsk.hr/urn:nbn:hr:178:723891

Butković I. Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2015 [cited 2025 April 03] Available at: https://urn.nsk.hr/urn:nbn:hr:178:723891

I. Butković, "Osjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:178:723891

Metadata
TitleOsjetljivost bakterija na antimikrobne tvari iz mlijeka zdravih i terapiranih vimena krava
Title (english)Antimicrobial Susceptibility of Bacteria Isolated from Milk of Healthy and Drug-Treated Cow Udder
AuthorIvan Butković
MentorNevijo Zdolec (mentor)
Committee memberVesna Dobranić
Committee memberIvana Filipović
Committee memberNevijo Zdolec
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Hygiene and Technology of Foodstuffs)
Zagreb
Defense date and country2015-12-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Cilj ovog rada bio je istražiti sastav mikroflore mlijeka krava te pojavnost otpornosti bakterija mlijeka na antimikrobne tvari s obzirom na zdravlje njihova vimena. Mlijeko je sterilno uzorkovano od krava koje nisu nikada pokazivale znakove mastitisa (n=17) te krava koje su bile terapirane i izliječene (n=19), a potjecale su s 4 različite farme. Normiranim mikrobiološkim metodama određivan je broj aerobnih mezofilnih bakterija, psihrofilnih bakterija, bakterija mliječne kiseline,... More stafilokoka, Escherichia coli, enterokoka, enterobakterija, Listeria spp. te sulfitreducirajućih klostridija, Testiranje osjetljivosti na antimikrobne lijekove provedeno je na ukupno 56 izolata enterokoka, 30 izolata Escherichia coli, 24 enterobakterija i 94 stafilokoka. Ovisno o bakterijskoj skupini ili vrsti u disk difuzijskom testu korišteni su diskovi ampicilina, rifampina, kloramfenikola, tetraciklina, eritromicina, nitrofurantoina, vankomicina, penicilina, linezolida, trimetoprima, cefoperazona, kanamicina, trimetoprim/sulfametoksazola, nalidiksične kiseline, ciprofloksacina, gentamicina, teicoplanina, sulfonamida, levofloksacina, klindamicina te amoksicilina s klavulanskom kiselinom. Statistički značajne razlike (p<0,05) s obzirom na podrijetlo uzoraka mlijeka utvrđene su u broju aerobnih mezofilnih bakterija i bakterija mliječne kiseline. Pojavnost višestruko otpornih izolata E. coli i stafilokoka bila je značajno veća (P<0.05) u mlijeku liječenih krava. Postotni odnos (liječene:zdrave krave) višestruko otpornih E. coli bio je 56,3:0, enterobakterija 57,14:30, te stafilokoka 56,5:4,8. Nije bilo značajne razlike (P>0.05) u pojavnosti rezistentnih enterokoka u mlijeku zdravih i liječenih krava (73,7 : 87.2 %). Visoka učestalost otpornosti na antimikrobne tvari enterokoka u mlijeku zdravih krava može biti posljedica kohabitacije životinja i križnog onečišćenja. Less
Abstract (english)
The aim of this study was to determine the composition of cow milk microbiota and antimicrobial resistance of milk microbiota in relation to udder health. Milk was aseptically sampled from healthy cows without any signs of mastitis (n=17) and drug-treated cows with mastitis (n=19), originated from 4 farms. Total viable count, psychrophilic bacteria, lactic acid bacteria, staphylococci, Escherichia coli, enterococci, enterobacteria, Listeria spp. and sulphite-reducing clostridia... More were determined using standard cultured methods. Antimicrobial susceptibility testing was performed in 56 enterococci, 30 Escherichia coli, 24 enterobacteria and 94 staphylococci. Depending of bacterial group or species, the following antibiotic disks were used – ampicillin, rifampin, chloramphenicol, tetracycline, erythromycin, nitrofurantoin, vancomycin, penicillin, linezolid, trimethoprim, cefoperazone, kanamycin, nitrofurantoin, trimethoprim/sulfamethoxazole, nalidixic acid, ciprofloxacin, gentamicin, teicoplanin, sulfonamides, levofloxacin, klindamycin and amoxicillin/clavulanic acid. Statistically significant differences (P<0,05) considering the origin of milk samples were found in total viable count and lactic acid bacteria. occurrence of multiresistant E. coli and staphylococci was significantly higher (P<0.05) in milk of drug-treated cows. The percentage ratio (drug-treated: healthy udders) of multiresistant E. coli was 56.3:0, enterobacteria 57.14:30 and staphylococci 56.5: 4.8. There was no significant difference (P>0.05) between the prevalence of multiresistant enterococci in the milk of healthy cows and cows with cured mastitis (73.7: 87.2). High prevalence of resistance in enterococci isolated from milk samples of healthy cows could be result of animal cohabitation and crosscontamination. Keywords: milk, udder health, anti Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:723891
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-01-18 09:53:59
accessibility

closeAccessibilityrefresh

If you wish to permanently save changes, click on Save, if not - your settings will be reset when you restart the browser.