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master's thesis
Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2020. urn:nbn:hr:178:543852
Rak, Jana
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

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Rak, J. (2020). Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:543852

Rak, Jana. "Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:178:543852

Rak, Jana. "Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:178:543852

Rak, J. (2020). 'Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:543852

Rak J. Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2020 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:178:543852

J. Rak, "Antimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:178:543852

Metadata
TitleAntimikrobni, antioksidativni i imunomodulacijski učinak tekućih ekstrakata propolisa
Title (english)ANTIMICROBIAL, ANTIOXIDANT AND IMMUNOMODULATORY EFFECT OF LIQUID PROPOLIS EXTRACTS
AuthorJana Rak
MentorJelena Šuran (mentor)
MentorJosipa Vlainić (mentor)
Committee memberFrane Božić (predsjednik povjerenstva)
Committee memberAndreja Prevendar Crnić (član povjerenstva)
Committee memberJelena Šuran (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2020-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Cilj ovog istraživanja bio je usporedba koncentracije deset markera biološke aktivnosti (biomarkera) u šest različitih tekućih ekstrakata propolisa, alkoholnih (EEP1-3) i bezalkoholnih (BEP1-3), s antimikrobnim i antioksidativnim učinkom. Koncentracija deset biomarkera utvrđena je metodom tekućinske kromatografije visoke djelotvornosti s UV-Vis detektorom (HPLC). U obje vrste otopina (EEP i BEP) najzastupljeniji biomarkeri jesu p-kumarinska i trans-ferulinska kiselina, dok su kempferol i trans-cimetna kiselina najmanje zastupljeni biomarkeri. Koncentracija najzastupljenijih biomarkera veća je u BEP-a, nego u EEP-a. Nije utvrđena povezanost koncentracije biomarkera i postotka otopljenog propolisa u obje vrste tekućih ekstrakata propolisa. Antimikrobni učinak šest ekstrakata propolisa određen je mjerenjem MIK-a i MBEK-a. Najbolji antimikrobni učinak (najniže vrijednosti MIK80 i MBEK) ima bezalkoholni ekstrakt BEP3. Provođenjem FRAP i DPPH metode određen je antioksidativni učinak ekstrakata propolisa. U ovom istraživanju BEP u odnosu na EEP, općenito su pokazali bolji antioksidativni učinak (osim BEP2).
Abstract (english)
The aim of this study was to compare the concentration of ten markers of biological activity (biomarkers) in six different liquid propolis extracts, alcoholic (EEP1-3) and non-alcoholic (BEP1-3), with antimicrobial and antioxidant effects. The concentration of ten biomarkers was determined by high performance liquid chromatography with a UV-Vis detector (HPLC). In both types of solutions (EEP and BEP) the most common biomarkers are p-coumaric and transferulic acid, while kaempferol and trans-cinnamic acid are the least represented biomarkers. The concentration of the most common biomarkers is higher in BEPs than in EEPs. No association was found between the biomarker concentration and the percentage of dissolved propolis in both types of liquid propolis extracts. The antimicrobial effect of six propolis extracts was determined by measuring MIK and MBEC. The best antimicrobial effect (lowest values of MIK80 and MBEC) was shown by non-alcoholic extract of BEP3. The antioxidant effect of propolis extracts was determined by performing FRAP and DPPH methods. In this study, BEP compared to EEP, generally showed a better antioxidant effect (except BEP2)
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:543852
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-08-22 09:51:28