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master's thesis
Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:820131
Hadžiomeragić, Esma
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Hadžiomeragić, E. (2023). Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:820131

Hadžiomeragić, Esma. "Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:820131

Hadžiomeragić, Esma. "Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:820131

Hadžiomeragić, E. (2023). 'Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:820131

Hadžiomeragić E. Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:820131

E. Hadžiomeragić, "Biološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:820131

Metadata
TitleBiološki učinak vodenog ekstrakta jagode (Fragaria ananassa × Duch.) na in vitro modelima probavnog sustava
Title (english)Biological effect of aqueous strawberry (Fragaria ananassa × Duch.) extract on in vitro models of digestive system
AuthorEsma Hadžiomeragić
MentorKsenija Durgo (mentor)
MentorAna Huđek Turković (pomoć pri izradi, neposredni voditelj)
MentorAnica Bebek Markovinović (pomoć pri izradi, neposredni voditelj)
Committee memberDanijela Bursać Kovačević (predsjednik povjerenstva)
Committee memberPredrag Putnik (član povjerenstva)
Committee memberKristina Radošević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2023-04-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Jagoda (Fragaria ananassa × Duch.) predstavlja bogat izvor biološki i farmakološki aktivnih polifenolnih spojeva koji imaju pozitivan učinak na zdravlje čovjeka. Cilj ovog rada je ispitati biološki učinak jagode na različitim biološkim sustavima uključujući humane stanične linije predstavnike mikroflore čovjeka (Escherichia coli, Lactobacillus fermentum, Staphylococcus aureus), i modelnu DNA, plazmid phiX174. U tu svrhu korištene su metode Neutral red za određivanje citotoksičnosti ekstrakta na humanim staničnim linijama, DCFH-DA za određivanje prooksidacijskog/antioksidacijskog djelovanja na humanim staničnim linijama, agarozna elektroforeza za određivanje genoprotektivnog učinka te protokol za utvrđivanje antimikrobnog djelovanja ekstrakta. Dobiveni rezultati pokazali su da vodeni ekstrakt jagode djeluje citotoksično na staničnu liniju adenokarcinoma debelog crijeva i karcinoma epitela usne šupljine. Ekstrakt jagode nije pokazao antioksidacijsko djelovanje niti na ijednoj staničnoj liniji. Utvrđen je i genoprotektivni učinak jagode na modelnoj DNA. Biološki aktivni spojevi u ekstraktu jagode pokazali su antimikrobnu aktivnost prema Gram-pozitivnim bakterijama, L. fermentum i S. aureus.
Abstract (english)
Strawberry (Fragaria ananassa × Duch.) is a source of biologically and pharmacologically active polyphenols that have beneficial effects on human health. The aim of this work is to investigate the biological effect of strawberry extract on different biological systems, including human cell lines, human microflora (Escherichia coli, Lactobacillus fermentum, Staphylococcus aureus), and model DNA, plasmid phiX174. Various methods were used, including Neutral red to determine cytotoxicity to human cell lines, DCFH-DA to determine prooxidant/antioxidant effect on human cells, agarose electrophoresis to determine genoprotective effect, and a protocol to determine antimicrobial effect of the extract. The results showed that the strawberry extract has a cytotoxic effect on adenocarcinoma cells of the colon and epithelial carcinoma cells of the oral cavity. The strawberry extract showed no antioxidant effect on any of the cell lines studied. The strawberry extract showed genoprotective effect on the model DNA. Biologically active compounds in the extract showed antimicrobial activity against Gram-positive bacteria, L. fermentum and S. aureus.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:820131
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-05-21 12:43:35