prikaz prve stranice dokumenta Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona
  Download
PDF 4 MB
doctoral thesis
Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. urn:nbn:hr:109:984267
Lončarić, Melita
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Applied Chemistry and Ecology
Sub-department of Chemistry and Ecology

Linked objects
Spektri masa i NMR spektri (1H i 13C) sintetiziranih kumarina, rodanina i tiazolidindiona | Appendix

Institutional repository: Repository of the Faculty of Food Technology Osijek

Cite this document

Lončarić, M. (2022). Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona (Doctoral thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:984267

Lončarić, Melita. "Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. https://urn.nsk.hr/urn:nbn:hr:109:984267

Lončarić, Melita. "Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2022. https://urn.nsk.hr/urn:nbn:hr:109:984267

Lončarić, M. (2022). 'Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona', Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:109:984267

Lončarić M. Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona [Doctoral thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2022 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:109:984267

M. Lončarić, "Inhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona", Doctoral thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:109:984267

Metadata
TitleInhibicija lipooksigenaze derivatima kumarina, rodanina i tiazolidindiona
Title (english)Lipoxygenase inhibition by coumarin, rhodanine and thiazolidinedione derivatives
AuthorMelita Lončarić
MentorMaja Molnar (mentor)
MentorValentina Pavić (komentor)
Committee memberDajana Gašo-Sokač (predsjednik povjerenstva)
Committee memberIvica Strelec (član povjerenstva)
Committee memberTatjana Gazivoda Kraljević (član povjerenstva)
Committee memberValentina Bušić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Applied Chemistry and Ecology)
(Sub-department of Chemistry and Ecology)
Osijek
Defense date and country2022-03-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
64 - Home economics. Domestic science. Housekeeping
Abstract
Cilj ovog rada bio je razviti zelenu metodu za sintezu derivata kumarina, rodanina i tiazolidindiona primjenom niskotemperaturnih eutektičkih otapala na bazi kolin-klorida te ispitati njihovu biološku aktivnost. Za svaku grupu spojeva odabrana je modelna reakcija koja je provedena u 20 različitih niskotemperaturnih eutektičkih otapala, te je na temelju najvišeg iskorištenja odabrano najpogodnije otapalo za daljnju sintezu. Za sintezu derivata kumarina najpogodnijim otapalom pokazao se kolin-klorid : urea, za sintezu derivata rodanina kolin-klorid : acetamid, a za sintezu taizolidindiona kolin-klorid : N-metilurea. Sinteza serije ciljanih derivata provedena je na temelju odabira odgovarajućeg otapala, konvencionalnom metodom uz miješanje, reakcijom potpomognutom mikrovalovima, te ultrazvukom. Nadalje, u radu je ispitana inhibicija aktivnosti enzima lipooksigenaze iz soje te inhibicija lipidne peroksidacije sintetiziranim spojevima. Spojevima je također određena antioksidacijska aktivnost ABTS i DPPH metodom. Aktivnost lipooksigenaze uspješno je inhibirana 7,1 – 96,9 % derivatima kumarina, 3,7 – 100,0 % derivatima rodanina i 7,3 – 76,3 % derivatima tiazolidindiona. Dobiveni rezultati upućuju da sintetizirani spojevi imaju potencijala za sprječavanje dioksigenacije kod višestruko nezasićenih masnih kiselina i nastajanja konjugiranih dienskih hidroperoksida u prehrambenim proizvodima.
Abstract (english)
The aim of this study was to develop a green method for the synthesis of coumarin, rhodanine and thiazolidinedione derivatives and to examine their biological activity. For each group of compounds, a model reaction was selected and performed in 20 different deep eutectic solvents in order to find the most suitable solvent for further synthesis based on the highest yield. The most suitable solvents for the synthesis of coumarin, rhodanine and thiazolidinedione derivatives were choline chloride : urea, choline chloride : acetamide and choline chloride : N-methylurea, respectively. After selecting the most suitable solvent, synthesis with stirring, microwave synthesis and ultrasound-assisted synthesis were performed. The synthesized compounds were tested for inhibition of lipid peroxidation as well as for inhibition of soybean lipoxygenase enzyme activity. The antioxidant activity of the compounds was also determined by the ABTS and DPPH method. Coumarin, rhodanine and thiazolidinedione derivatives successfully inhibited lipoxygenase activity with inhibition ranging 7.1 - 96.9%, 3.7 - 100.0% and 7.3 - 76.3%, respectively. According to the obtained results, it can be concluded that the synthesized compounds have the potential to prevent dioxygenation in polyunsaturated fatty acids and the formation of conjugated diene hydroperoxides in food products.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:984267
Promotion2022
ProjectNumber: UIP-2017-05-6593
Title: Zelene tehnologije u sintezi heterocikličkih spojeva
Acronym: GREENNESS
Leader: Maja Molnar
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: UIP
Study programmeTitle: FOOD TECHNOLOGY AND NUTRITION
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor znanosti (dr. sc.) iz znanstvenog područja Biotehničkih znanosti, znanstvenog polja Prehrambena tehnologija (doktor znanosti (dr. sc.) iz znanstvenog područja Biotehničkih znanosti, znanstvenog polja Prehrambena tehnologija)
Catalog URLhttp://161.53.208.100/cgi-bin/lb10/unilib.cgi?form=D1580157001
Type of resourceText
Extent189 str.: ilustr., graf. prikazi
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) icon
Created on2022-06-24 10:42:25